生物技术通报 ›› 2019, Vol. 35 ›› Issue (9): 70-74.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0331

• 技术与方法 • 上一篇    下一篇

包埋法固定钩状木霉孢子还原水体中六价铬的研究

王文婧, 卢松霖, 刘雪, 李司宇, 郝雪萌, 李媛媛, 张杰   

  1. 东北林业大学生命科学学院,哈尔滨 150040
  • 收稿日期:2019-04-16 出版日期:2019-09-26 发布日期:2019-09-16
  • 作者简介:王文婧,女,研究方向:生物技术;E-mail:710855095@qq.com
  • 基金资助:
    国家基础科学人才培养基金资助项目(J1210053),东北林业大学创新资助项目(201810225499; 201910225497)

Reducing Cr(VI)from Water System via Immobilized Trichoderma hamatum Spores by Embedding Method

WANG Wen-jing, LU Song-lin, LIU Xue, LI Si-yu, HAO Xue-meng, LI Yuan-yuan, ZHANG Jie   

  1. College of Life Sciences,Northeast Forestry University,Harbin 150040
  • Received:2019-04-16 Published:2019-09-26 Online:2019-09-16

摘要: 旨在探索固定化微生物还原六价铬的新方法,给后期固定化微生物还原六价铬提供新的研究思路。利用包埋法制备两种钩状木霉BSL01孢子包埋小球PVA-SA和PVA-SA-PDA,进一步研究两种小球的性能差异,如对水体中六价铬的还原效率以循环利用次数。结果表明,PVA-SA包埋小球对六价铬的还原效率为93.4%,可重复利用18次;PVA-SA-PDA固定小球还原六价铬效率为97.2%,可重复利用23次。两种固定化小球从第8个循环开始,3 d可以完全还原浓度为50 mg/L的六价铬,接近游离菌体还原六价铬效率的2倍。该研究首次将PDA培养基和真菌孢子制备成核壳小球,小球循环利用次数增多,且效果良好。

关键词: 包埋法, 钩状木霉, 六价铬

Abstract: This paper aims to explore a novel method of reducing hexavalent chromium by immobilized microorganisms and to provide a new idea for the further research. Spore-encapsulated microspheres PVA-SA and PVA-SA-PDA with Trichoderma hamatum BSL01 were prepared by embedding method. The performance variation of PVA-SA and PVA-SA-PDA microspheres,such as the reduction efficiency of Cr(VI)and the recycling times,were further studied. The results showed that the reduction efficiency of Cr(VI)by PVA-SA immobilized pellets was 93.4%,and it could be reused 18 cycles. The reduction efficiency of PVA-SA-PDA pellets was 97.2%,and it could be reused 23 cycles. Cr(VI)with a concentration of 50 mg/L was completely reduced using PVA-SA and PVA-SA-PDA in 3 d from the 8th cycle,which was nearly twice as that of free bacteria in reducing Cr(VI). It is the first time that PDA medium and fungal spores are prepared into nucleocapsid microspheres that are recycled more times and have fine results.

Key words: embedding method, Trichoderma hamatum, Cr(VI)