肠道菌群在应激诱发大鼠高血压中的作用

doi:10.13560/j.cnki.biotech.bull.1985.2019-1215

摘要/Abstract

摘要： 观察应激性高血压大鼠肠道菌群的变化,探索肠道菌群在应激性高血压发生中的作用。应激性高血压模型的制备：大鼠每天2次接受足底电刺激加噪声的应激,每次2 h,中间间隔4 h,共14 d。血压的测量：大鼠股动脉插管,Powerlab系统记录血压。伪无菌鼠的制备：在大鼠的饮用水中加入氨苄西林（1 g/L）、万古霉素（500 mg/L）、新霉素（1 g/L）和甲硝唑（1 g/L）4种抗生素,共持续4周。采用 Illumina MiSeq 测序技术,测定大鼠粪便中微生物16S rRNA V3-V4 区序列,并对群落结构和多样性进行比较分析。慢性应激导致大鼠血压升高,心率加快,血清去甲肾上腺素浓度增加;α多样性分析显示应激大鼠肠道菌群丰富度和多样性下降,β多样性分析显示应激组大鼠肠道菌群物种组成变少,差异性变大;伪无菌鼠的肠道菌群物种单一,仅有少量的变形菌门。给予伪无菌鼠相同的慢性应激,不能引起大鼠血压升高、心率增快,血清去甲肾上腺素浓度未升高。应激引起大鼠肠道菌群紊乱,应激性高血压的发生可能依赖于肠道菌群的状态。

关键词: 应激, 高血压, 肠道菌群, 大鼠

Abstract: This work is to observe the variation of gut microbiota in stress-induced hypertension in rats and to investigate the role of gut microbiota in the pathogenesis of stress-induced hypertension in rats. The model of stress-induced hypertension rats was prepared by：Rats were subjected to plantar electrical stimulation and noise twice a day for 2 h at an interval of 4 h for consecutive 14 d. Measurement of blood pressure was conducted by：arterial catheterization in rats,and then blood pressure was recorded by Powerlab system. The model of pseudo germ-free rats was prepared by adding 4 antibiotics of 1 g/L ampicillin,500 mg/L vancomycin,1 g/L neomycin,1 g/L metronidazole file in their drinking water for 4 weeks. The 16S rRNA V3-V4 region sequence of microbe in rat feces was determined by Illumina MiSeq sequencing technique,and the community structure and diversity were compared and analyzed. Chronic stress increased blood pressure,heart rate and serum norepinephrine concentration in rats. α diversity analysis showed that the richness and diversity of gut microbiota decreased in stress rats,and β diversity analysis showed that the species composition of gut microbiota in stress group decreased and the difference became greater. In the pseudo germ-free rats,the gut microbiota species was simple with only a small number of Proteobacteria and the chronic stress did not increase blood pressure,heart rate as well as serum norepinephrine concentration. Stress may cause disorders of gut microbiota in rats and stress-induced hypertension may depend on gut microbiota.

Key words: stress, hypertension, gut microbiota, rat

吴芹, 许子洋, 刘丽萍, 张文英, 宋思远. 肠道菌群在应激诱发大鼠高血压中的作用[J]. 生物技术通报, 2020, 36(2): 83-90.

WU Qin, XU Zi-yang, LIU Li-ping, ZHANG Wen-ying, SONG Si-yuan. Role of Gut Microbiota in Stress-induced Hypertension in Rats[J]. Biotechnology Bulletin, 2020, 36(2): 83-90.

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