乙醇对瘤胃液接种稻秸的体外发酵产物及细菌群落结构的影响

doi:10.13560/j.cnki.biotech.bull.1985.2019-1101

摘要/Abstract

摘要： 旨在研究乙醇对山羊瘤胃液与水稻秸秆厌氧共培养的影响。利用频繁传代的体外发酵技术和高通量测序方法,分析了短链脂肪酸（SCFA）产量和细菌群落的变化。结果表明,经体外培养传代8次的稻秸发酵液的总短链脂肪酸产量显著高于瘤胃液（P<0.01）;与未添加乙醇的稻秸发酵液相比,添加乙醇显著提高了乙酸、戊酸和己酸的比例,降低了丙酸和丁酸的比例（P<0.01）,总SCFA产量及异丁酸和异戊酸比例无显著差异。与瘤胃液相比,稻秸发酵液的拟杆菌门（Bacteroidetes）相对丰度下降,厚壁菌门（Firmicutes）相对丰度升高（P<0.05）,且添加乙醇显著提高了厚壁菌门和放线菌门（Actinobacteria）的相对丰度（P<0.05）;添加乙醇使双歧杆菌属（Bifidobacterium）、未定性的毛螺菌属（unidentified Lachnospiraceae）、产琥珀酸菌属（Succiniclasticum）、脱硫弧菌属（Desulfovibrio）和未定性的梭菌属（unidentified Clostridiales）的相对丰度显著升高（P<0.05）。乙醇使稻秸发酵液的显著性差异物种（Biomarker）增加;稻秸发酵液与瘤胃液亲缘关系较近,而添加乙醇显著改变了细菌区系;短链脂肪酸比例在稻秸发酵液细菌群落多样性中具有重要作用。研究表明,体外频繁传代和添加乙醇可以提高稻秸发酵液的乙酸、戊酸和己酸产量,乙醇改变了稻秸发酵液的细菌群落结构。

关键词: 乙醇, 瘤胃液, 稻秸, 短链脂肪酸, 细菌群落

Abstract: This study aims to study the effect of ethanol addition on rice straw anaerobic fermentation co-cultured with rumen fluid. In vitro fermentation technique by frequent passage and high-throughput sequencing technology were applied to analyze short chain fatty acid（SCFA）yield and bacterial community composition. The results showed that SCFA yield in the rice straw fermentation by in vitro 8^th transferring were significant higher than that by rumen fluid（P<0.01）. Compared with no adding ethanol,ethanol addition significantly increased the proportion of acetate,valerate,and caporate,while decreased propoinate and butyrate proportion（P<0.01）;the total SCFA yield,the proportion of isobutyrate and isovelarate varied insignificantly. The relative abundance of Bacteroidetes phylum decreased,while that of Firmicutes phylum increased by anaerobic fermentation of rice straw（P<0.05）,compared with that by rumen fluid. Furthermore,the relative abundances of Firmicutes and Actinobacteria phyla,Bifidobacterium,unidentified Lachnospiraceae,Succiniclasticum,Desulfovibrio and unidentified Clostridiales genera increased while ethanol was added（P<0.05）. Adding ethanol increased Biomarkers in the rice straw fermentation. The rice straw fermentation and rumen fluid presented close genetic relationship,and adding ethanol significantly changed the bacterial community structure. The proportion of SCFA played an important role in bacterial community of the rice straw fermentation. Our results indicate that in vitro frequent passage and adding ethanol increase the proportion of acetate,valerate,and caporate in rice straw fermentation as ethanol changes the bacterial community of rice straw fermentation.

Key words: ethanol, rumen fluid, rice straw, short chain fatty acid, bacterial community

林淼, 王阔鹏, 陈映良, 孙文婧, 封丽梅, 胡梓轩. 乙醇对瘤胃液接种稻秸的体外发酵产物及细菌群落结构的影响[J]. 生物技术通报, 2020, 36(2): 91-99.

LIN Miao, WANG Kuo-peng, CHEN Ying-liang, SUN Wen-jing, FENG Li-mei, HU Zi-xuan. Effects of Ethanol on Metabolites and Bacterial Community of Rice Straw Cocultured with Rumen Fluid in vitro[J]. Biotechnology Bulletin, 2020, 36(2): 91-99.

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