氯氰菊酯降解菌草酸青霉SSCL-5分离鉴定及降解特性

doi:10.13560/j.cnki.biotech.bull.1985.2019-1072

摘要/Abstract

摘要： 为解决土壤中残留的菊酯类农药问题,通过富集筛选法从70余份土壤中获得一株高效分解利用菊酯类农药的微生物菌株SSCL-5,该菌株可在含1 000 mg/L的氯氰菊酯无机盐培养基中正常生长。经形态学及ITS测序鉴定,确定该菌为草酸青霉（Penicillium oxalicum）,对多种高浓度菊酯类农药耐受。经紫外分光光度法及HPLC验证该菌株草酸青霉SSCL-5在无机盐培养基、28℃、180 r/min摇瓶培养24 h的条件下,对400 mg/L氯氰菊酯的降解率为97%。土壤室内试验证明该菌在土壤中,温度20-34℃、水分含量保藏40%-60%条件下,30 d可将土壤中400 mg/L的氯氰菊酯降解67.6%。

关键词: 真菌, 氯氰菊酯, 草酸青霉, 生物降解

Abstract: To find a solution of removing pyrethroid pesticide residues in soil,enrichment and screening methods were used,and a microbial strain,which efficiently decomposed and utilized pyrethroid-like pesticides,was isolated from over 70 soil samples,and named as SSL-5. The strain grew normally in the mineral medium containing 1 000 mg/L cypermethrin. According to the results of morphology,ITS Sequencing analysis,the strain SSCL-5 was identified as Penicillium oxalicum,and tolerant to other several pyrethroid-like pesticides in high concentration. Degradation rate of cypermethrin（400 mg/L）by this SSL-5 in the mineral medium at 28℃ and 180 r/min and culturing 24 h was verified to be 97% with HPLC and UV spectrophotometry. Laboratory soil tests proved that cypermethrin in soil（400 mg/L）was degraded by 67.6% in 30 d while temperature in 20℃-34℃ and moisture content in 40%-60%.

Key words: fungi, cypermethrin, Penicillium oxalicum, biodegradation

陈锐, 瞿佳, 孙晓宇, 邓媛, 门欣, 赵玲侠, 沈卫荣. 氯氰菊酯降解菌草酸青霉SSCL-5分离鉴定及降解特性[J]. 生物技术通报, 2020, 36(6): 120-127.

CHEN Rui, QU Jia, SUN Xiao-yu, DENG Yuan, MEN Xin, ZHAO Ling-xia, SHEN Wei-rong. Isolation and Identification of Penicillium oxalicum SSCL-5 Degrading Cypermethrin and Its Biodegradation[J]. Biotechnology Bulletin, 2020, 36(6): 120-127.

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