微生物碳酸酐酶诱导CaCO3沉淀的影响因素及生成机理

doi:10.13560/j.cnki.biotech.bull.1985.2019-1218

摘要/Abstract

摘要： 微生物碳酸酐酶能够加速CO₂的水合反应,对于研究全球碳循环具有重要的意义。选用一种产生胞外碳酸酐酶的细菌,研究了温度、pH值和Ca²⁺浓度对细菌生长、碳酸酐酶活性的影响。结果表明,25℃碳酸酐酶的活性最高,有利于CaCO₃的沉淀;初始pH值为8.5的偏碱性环境中,CaCO₃沉淀质量最多;当Ca²⁺浓度为50 mmol/L时,细菌的生长繁殖最好,过低的Ca²⁺浓度会影响CaCO₃的生成,而过高的Ca²⁺浓度则会严重影响细菌的生长,降低细菌的活性。最后研究了微生物碳酸酐酶诱导CaCO₃沉淀的机理,碳酸酐酶能够加速CO₂水化成HCO₃^-,在碱性环境中、钙源存在的情况下,与OH^-和Ca²⁺反应生成CaCO₃沉淀。

关键词: 微生物, 碳酸酐酶, 酶活性, CaCO₃, 机理

Abstract: Microbial carbonic anhydrase can accelerate the CO₂ hydration,which is of great significance for the study of global carbon cycle. A bacterium producing extracellular carbonic anhydrase was selected to study the effects of temperature,pH value and Ca²⁺ concentration on bacterial growth and carbonic anhydrase activity in this article. The results showed that the activity of carbonic anhydrase at 25℃ was the highest,which was beneficial to the CaCO₃ precipitation. The precipitation of CaCO₃ was the most in the alkaline environment with the initial pH 8.5. When the Ca²⁺ concentration was 50 mmol/L,the growth and reproduction of the bacterium were the best,too low Ca²⁺ concentration affected the generation of CaCO₃,while too high Ca²⁺concentration seriously affected the growth of the bacterium and reduced the activity of the bacterium. Finally,the mechanism of CaCO₃ precipitation induced by microbial carbonic anhydrase was studied. Carbonic anhydrase accelerated the hydration of CO₂ into HCO₃^-,and HCO₃^-reacted with OH^-and Ca²⁺ to form CaCO₃ precipitation in alkaline environment and in the presence of calcium source.

Key words: microbial, carbonic anhydrase, enzymatic activity, CaCO₃, mechanism

袁亮. 微生物碳酸酐酶诱导CaCO₃沉淀的影响因素及生成机理[J]. 生物技术通报, 2020, 36(8): 79-68.

YUAN Liang. Influencing Factors and Formation Mechanism of CaCO₃ Precipitation Induced by Microbial Carbonic Anhydrase[J]. Biotechnology Bulletin, 2020, 36(8): 79-68.

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微生物碳酸酐酶诱导CaCO₃沉淀的影响因素及生成机理

Influencing Factors and Formation Mechanism of CaCO₃ Precipitation Induced by Microbial Carbonic Anhydrase

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