樟叶越桔嫩枝1株内生真菌的鉴定及抑菌活性测定

doi:10.13560/j.cnki.biotech.bull.1985.2020-0200

摘要/Abstract

摘要：

从樟叶越桔嫩枝中分离得到1株内生真菌Z-78,通过形态鉴别和分子生物学方法,鉴定为木防己拟盘多毛孢（Pestalotiopsis cocculi（Guba）G.C. Zhao & N. Li）。采用平板对峙法测定了其对灰葡萄孢、腐皮镰孢、禾谷镰孢、尖孢镰孢、芸苔链格孢、密粘褶菌、绵腐卧孔菌和采绒革盖菌等8种植物病原菌的抑制活性,采用生长速率法对其发酵液抑制芸苔链格孢活性进行了酸碱稳定性试验。结果表明：内生真菌Z-78对8种供试病原菌均有较强的抑菌活性,其发酵液在不同pH条件下对芸苔链格孢保持活性稳定。

关键词: 樟叶越桔, 嫩枝, 内生真菌, 木防己拟盘多毛孢, 抑菌活性

Abstract:

An endophytic fungal strain Z-78 isolated from the twigs of Vaccinium dunalianum Wight was identified as Pestalotiopsis cocculi（Guba）G.C. Zhao & N. Li by morphological classification and molecular biological study. The plate confrontation method was used to determine its antifungal activities against eight plant pathogens including Botrytis cinerea,Fusarium solani,Fusarium graminearum,Fusarium oxysporum,Alternaria brassicicola,Gloeophyllum trabeum,Poria placenta and Coriolus versicolor,and the growth rate method was applied to conduct the acid-base stability test of the fermentation broth inhibiting the activity of A. brassicicola. The results showed that Z-78 strain presented strong inhibitory effects on all of the eight plant pathogens,while the activity against A. brassicicola remained stable under different pH conditions of fermentation broth.

Key words: Vaccinium dunalianum, twig, endophytic fungi, Pestalotiopsis cocculi, antifungal activity

严冬, 曾为林, 罗旭璐, 陈肖学, 刘惠民, 赵平. 樟叶越桔嫩枝1株内生真菌的鉴定及抑菌活性测定[J]. 生物技术通报, 2020, 36(11): 30-38.

YAN Dong, ZENG Wei-lin, LUO Xu-lu, CHEN Xiao-xue, LIU Hui-min, ZHAO Ping. Identification and Antifungal Activity Determination of an Endophytic Fungus from the Twigs of Vaccinium dunalianum[J]. Biotechnology Bulletin, 2020, 36(11): 30-38.

图/表 6

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病原真菌	生长直径/mm	抑菌率/%	抑菌带宽度/mm	抑菌指数
密粘褶菌	25±1.8	72.22	0.00	+++
绵腐卧孔菌	25±1.3	70.39	3.00	+++
尖孢镰孢	32±2.4	61.76	0.00	+++
禾谷镰孢	30±2.5	61.11	0.00	+++
芸苔链格孢	35±2.0	61.00	3.00	+++
采绒革盖菌	35±1.4	57.84	0.00	+++
腐皮镰孢	41±1.9	52.35	0.00	+++
灰葡萄孢	42±2.8	50.00	0.00	+++

病原真菌	生长直径/mm	抑菌率/%	抑菌带宽度/mm	抑菌指数
密粘褶菌	25±1.8	72.22	0.00	+++
绵腐卧孔菌	25±1.3	70.39	3.00	+++
尖孢镰孢	32±2.4	61.76	0.00	+++
禾谷镰孢	30±2.5	61.11	0.00	+++
芸苔链格孢	35±2.0	61.00	3.00	+++
采绒革盖菌	35±1.4	57.84	0.00	+++
腐皮镰孢	41±1.9	52.35	0.00	+++
灰葡萄孢	42±2.8	50.00	0.00	+++

pH	2 d		4 d		6 d		8 d		10 d
pH	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%
2.0	5.1±0.38	47.9	9.7±0.38	49.6	13.3±0.25	54.4	17.0±0.25	55.7	19.9±0.14	53.1
4.0	5.4±0.14	44.4	9.4±0.29	50.9	13.0±0.43	55.3	16.3±0.14	57.5	18.5±1.15	56.5
6.0	5.5±0.25	43.6	9.2±0.14	52.2	12.8±0.29	55.9	16.1±0.29	58.1	19.2±0.14	54.9
8.0	6.3±0.25	35.9	10.8±0.10	43.9	14.8±0.38	49.0	18.2±0.14	52.7	22.4±0.18	47.4
10.0	5.2±0.14	47.0	8.9±0.14	53.5	12.3±0.29	57.6	15.4±0.14	59.9	18.7±0.14	56.1

pH	2 d		4 d		6 d		8 d		10 d
pH	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%
2.0	5.1±0.38	47.9	9.7±0.38	49.6	13.3±0.25	54.4	17.0±0.25	55.7	19.9±0.14	53.1
4.0	5.4±0.14	44.4	9.4±0.29	50.9	13.0±0.43	55.3	16.3±0.14	57.5	18.5±1.15	56.5
6.0	5.5±0.25	43.6	9.2±0.14	52.2	12.8±0.29	55.9	16.1±0.29	58.1	19.2±0.14	54.9
8.0	6.3±0.25	35.9	10.8±0.10	43.9	14.8±0.38	49.0	18.2±0.14	52.7	22.4±0.18	47.4
10.0	5.2±0.14	47.0	8.9±0.14	53.5	12.3±0.29	57.6	15.4±0.14	59.9	18.7±0.14	56.1