生物技术通报 ›› 2021, Vol. 37 ›› Issue (2): 129-137.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0471
收稿日期:
2020-04-22
出版日期:
2021-02-26
发布日期:
2021-02-26
作者简介:
王珊珊,女,硕士研究生,研究方向:中药药剂学;E-mail: 基金资助:
WANG Shan-shan(), SUN Min, WANG Yong-xia, LI Wei-dong, HAN Chun-chao()
Received:
2020-04-22
Published:
2021-02-26
Online:
2021-02-26
摘要:
对鸡腿蘑多糖的结构进行检测,并在此基础上探讨结构与活性的关系,对深度发掘鸡腿蘑多糖的功效具有重要意义。制备发酵时间为72 h、96 h和120 h的鸡腿蘑胞外粗多糖,采用PMP柱前衍生化-HPLC法分析其单糖组成,结果表明发酵72 h、96 h和120 h胞外多糖均由D-甘露糖、L-鼠李糖、D-葡萄糖、D-半乳糖、D-阿拉伯糖组成,但各单糖的相对摩尔比不同。采用扫描电子显微镜和原子力显微镜对不同发酵时间的多糖形貌结构进行分析,结果显示发酵120 h多糖的板状结构更加规则,多糖分子的聚集作用更强。采用高效凝胶过滤色谱法对不同发酵时间的鸡腿蘑胞外多糖的分子量分布情况进行分析,结果显示发酵120 h的多糖分子量更大,分布范围更广。体外抗氧化试验结果显示,在相同浓度下,发酵120 h多糖的抗氧化活性较发酵72 h和96 h的多糖强。研究结果表明鸡腿蘑胞外多糖的抗氧化活性可能与其形貌结构和分子量分布有关。
王珊珊, 孙敏, 王永霞, 李惟栋, 韩春超. 鸡腿蘑胞外多糖的形貌结构及分子量动态变化与抗氧化的相关性研究[J]. 生物技术通报, 2021, 37(2): 129-137.
WANG Shan-shan, SUN Min, WANG Yong-xia, LI Wei-dong, HAN Chun-chao. Correlation Detween the Dynamic Changes of Morphological Structure and Molecular Weight of Coprinus comatus Extracellular Polysaccharides and Their Antioxidant Activity[J]. Biotechnology Bulletin, 2021, 37(2): 129-137.
单糖 | 回归方程 | 相关系数 |
---|---|---|
D-甘露糖D-mannose | y=182277x-127844 | R2=0.999 8 |
L-鼠李糖L-Rhamnose | y=155433x-101943 | R2=0.999 2 |
D-葡萄糖D-glucose | y=98305x-22473 | R2=0.999 7 |
D-半乳糖D-galactose | y=47785x+33756 | R2=0.999 2 |
D-阿拉伯糖Arabinose | y=78214x-112323 | R2=0.999 6 |
表1 五种单糖的线性范围和回归方程
单糖 | 回归方程 | 相关系数 |
---|---|---|
D-甘露糖D-mannose | y=182277x-127844 | R2=0.999 8 |
L-鼠李糖L-Rhamnose | y=155433x-101943 | R2=0.999 2 |
D-葡萄糖D-glucose | y=98305x-22473 | R2=0.999 7 |
D-半乳糖D-galactose | y=47785x+33756 | R2=0.999 2 |
D-阿拉伯糖Arabinose | y=78214x-112323 | R2=0.999 6 |
发酵时间/h | 单糖组成 | 摩尔比 |
---|---|---|
72 | 甘露糖-鼠李糖-葡萄糖-半乳糖-阿拉伯糖 | 0.019∶0.052∶0.137∶0.703∶0.183 |
96 | 甘露糖-鼠李糖-葡萄糖-半乳糖-阿拉伯糖 | 0.013∶0.036∶0.246∶1.010∶0.166 |
120 | 甘露糖-鼠李糖-葡萄糖-半乳糖-阿拉伯糖 | 0.028∶0.051∶0.183∶0.646∶0.156 |
表2 鸡腿蘑胞外多糖的单糖组成及摩尔比
发酵时间/h | 单糖组成 | 摩尔比 |
---|---|---|
72 | 甘露糖-鼠李糖-葡萄糖-半乳糖-阿拉伯糖 | 0.019∶0.052∶0.137∶0.703∶0.183 |
96 | 甘露糖-鼠李糖-葡萄糖-半乳糖-阿拉伯糖 | 0.013∶0.036∶0.246∶1.010∶0.166 |
120 | 甘露糖-鼠李糖-葡萄糖-半乳糖-阿拉伯糖 | 0.028∶0.051∶0.183∶0.646∶0.156 |
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