生物技术通报 ›› 2021, Vol. 37 ›› Issue (4): 145-154.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1060

• 研究报告 • 上一篇    下一篇

锑在斑马鱼不同组织中的积累及其对抗氧化系统的影响

徐琨1(), 杨爱江1,2,3(), 胡霞1,2,3, 邹海洮1, 李彬1, 刘吉1   

  1. 1.贵州大学资源与环境工程学院,贵阳 550025
    2.贵州大学环境工程规划设计研究院,贵阳 550025
    3.贵州喀斯特环境生态系统教育部野外科学观测研究站,贵阳 550025
  • 收稿日期:2020-08-21 出版日期:2021-04-26 发布日期:2021-05-13
  • 作者简介:徐琨,女,硕士研究生,研究方向:环境污染与控制;E-mail:764304931@qq.com
  • 基金资助:
    贵州省教育厅省级重点学科建设项目(黔学位合字ZDXK[2016]11);贵州省国内生态学一流学科建设项目(GNYL[2017]007);贵州省科技计划项目(黔科合基础[2020]1Y195)

Antimony Accumulation and Its Effect on Antioxidation System in Different Tissues of Danio rerio

XU Kun1(), YANG Ai-jiang1,2,3(), HU Xia1,2,3, ZOU Hai-tao1, LI Bin1, LIU Ji1   

  1. 1. Resources and Environmental Engineering College,Guizhou University,Guiyang 550025
    2. Institute of Environmental Engineering Planning and Designing,Guizhou University,Guiyang 550025
    3. Guizhou Karst Environmental Ecosystems Observation and Research Station,Ministry of Education,Guiyang 550025
  • Received:2020-08-21 Published:2021-04-26 Online:2021-05-13

摘要:

研究在不同Sb浓度胁迫下,斑马鱼各组织中Sb的积累以及各类酶活性及含量变化。以斑马鱼为受试对象,暴露于Sb溶液(0、13.26、26.53、39.79 mg/L)中14 d后,测定各组织Sb积累量、SOD、CAT活性及GSH、MDA含量。结果显示Sb在各组织中积累顺序为肝脏>鳃>肌肉>脑,且随着Sb胁迫浓度增大而逐渐增多。随着Sb胁迫浓度增高,肝脏、脑、鳃、肌肉SOD活性呈现不同变化趋势;CAT活性及GSH含量均呈先上升后下降的趋势,表现为低浓度促进,高浓度抑制;MDA含量呈逐渐升高的趋势。综上表明,Sb可在斑马鱼不同组织积累,激活机体抗氧化防御系统,并使机体产生脂质过氧化。

关键词: 锑, 斑马鱼, 积累, 抗氧化, 脂质过氧化

Abstract:

This study aimed to study the accumulation of antimony in zebrafish tissues and changes in various enzyme activities and contents under the stress of different concentration of antimony. Danio rerio were exposed to antimony solution(0,13.26,26.53,and 39.79 mg/L)for 14 d to determine the accumulation of antimony,the activity of superoxide dismutase(SOD),catalase(CAT),content of glutathione(GSH)and malondialdehyde(MDA)in each tissue. The results showed that the order of antimony accumulation in each tissue was as:Liver > Gill> Muscle > Brain and it gradually increased with the increase of antimony stress concentration. With the increased of antimony stress concentration,SOD activity in the liver,brain,gills and muscles presented different trends;CAT activity and GSH content both increased first and then decreased,which was as promotion at low concentration and inhibition at high concentration;MDA content tended gradually increasing. In conclusion,antimony can accumulate in different tissues of Danio rerio,activating antioxidant defense system and causing lipid peroxidation.

Key words: antimony, zebrafish, accumulation, antioxidant, lipid peroxidation