生物技术通报 ›› 2021, Vol. 37 ›› Issue (9): 68-76.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1104
• 青贮微生物专题(专题主编:杨富裕 教授) • 上一篇 下一篇
姜富贵(), 成海建, 魏晨, 张召坤, 苏文政, 时光, 宋恩亮()
收稿日期:
2021-08-26
出版日期:
2021-09-26
发布日期:
2021-10-25
作者简介:
姜富贵,男,博士,助理研究员,研究方向:反刍动物营养;E-mail: 基金资助:
JIANG Fu-gui(), CHENG Hai-jian, WEI Chen, ZHANG Zhao-kun, SU Wen-zheng, SHI Guang, SONG En-liang()
Received:
2021-08-26
Published:
2021-09-26
Online:
2021-10-25
摘要:
本试验旨在研究糖蜜添加量对杂交构树青贮营养价值、发酵品质和微生物多样性的影响。试验分为4个组,分别为无添加组(CK组),糖蜜添加量为5 g/kg组(M5组)、10 g/kg组(M10组)和20 g/kg组(M20组),每组4个重复,发酵时间为60 d。结果表明:随糖蜜添加量的增加,杂交构树青贮的干物质和粗蛋白质含量显著线性增加(P < 0.05),中性洗涤纤维和酸性洗涤纤维含量显著线性降低(P < 0.05)。随糖蜜添加量的增加,杂交构树青贮的乳酸含量显著线性增加(P = 0.002),pH值、丁酸含量和氨态氮/总氮显著线性降低(P < 0.05)。在门水平上,杂交构树青贮的优势菌门为厚壁菌门,其次为变形菌门;在属水平上,M10组和M20组乳杆菌属的比例较CK组和M5组显著增加(P = 0.005),魏斯氏菌属的比例显著降低(P = 0.003);M5组、M10组和M20组Clostridium_sensu_stricto_12和肠杆菌属的比例较CK组显著降低(P < 0.05)。综上所述,添加糖蜜对杂交构树青贮中的营养价值和发酵品质均有不同程度的提升作用,并可增加乳杆菌属的丰度,同时降低Clostridium_sensu_stricto_12和肠杆菌属有害微生物的数量。本试验条件下,添加20 g/kg糖蜜杂交构树青贮的营养价值和发酵品质最优。
姜富贵, 成海建, 魏晨, 张召坤, 苏文政, 时光, 宋恩亮. 糖蜜添加量对杂交构树青贮发酵品质和微生物多样性的影响[J]. 生物技术通报, 2021, 37(9): 68-76.
JIANG Fu-gui, CHENG Hai-jian, WEI Chen, ZHANG Zhao-kun, SU Wen-zheng, SHI Guang, SONG En-liang. Effects of Addition Amount of Molasses on the Fermentation Quality and Microbial Diversity of Hybrid Broussonetia papyrifera L. Vent Silage[J]. Biotechnology Bulletin, 2021, 37(9): 68-76.
项目Item | 含量Content/% |
---|---|
干物质DM | 28.73 |
粗蛋白CP | 17.69 |
中性洗涤纤维NDF | 44.80 |
酸性洗涤纤维ADF | 23.07 |
酸性洗涤木质素ADL | 8.70 |
粗灰分Ash | 11.70 |
粗脂肪EE | 6.49 |
水溶性碳水化合物WSC | 1.27 |
表1 杂交构树原料的营养成分(干物质基础)
Table 1 Nutritional composition of hybrid B. papyrifera L. Vent(dry matter basis)
项目Item | 含量Content/% |
---|---|
干物质DM | 28.73 |
粗蛋白CP | 17.69 |
中性洗涤纤维NDF | 44.80 |
酸性洗涤纤维ADF | 23.07 |
酸性洗涤木质素ADL | 8.70 |
粗灰分Ash | 11.70 |
粗脂肪EE | 6.49 |
水溶性碳水化合物WSC | 1.27 |
项目Item | 不同处理组Treatment | 标准误SEM | P值P value | ||||||
---|---|---|---|---|---|---|---|---|---|
CK | M5 | M10 | M20 | T | L | Q | C | ||
干物质DM/% | 24.41b | 26.83a | 28.02a | 28.32a | 0.466 | 0.001 | <0.001 | 0.027 | 0.898 |
粗蛋白质CP/% | 15.18b | 16.38ab | 16.98ab | 17.64a | 0.360 | 0.047 | 0.020 | 0.889 | 0.282 |
中性洗涤纤维NDF/% | 37.56a | 35.42a | 32.13b | 31.39b | 0.718 | <0.001 | <0.001 | 0.034 | 0.237 |
酸性洗涤纤维ADF/% | 20.88a | 20.48a | 19.41a | 17.41b | 0.403 | <0.001 | <0.001 | 0.490 | 0.658 |
酸性洗涤木质素ADL/% | 5.97 | 6.40 | 5.35 | 4.64 | 0.294 | 0.161 | 0.149 | 0.612 | 0.321 |
粗灰分Ash/% | 12.63a | 11.80b | 11.78b | 11.53b | 0.139 | 0.009 | 0.004 | 0.078 | 0.217 |
粗脂肪EE/% | 6.96 | 7.98 | 7.67 | 7.58 | 0.367 | 0.828 | 0.733 | 0.510 | 0.592 |
水溶性碳水化合物WSC/% | 0.54c | 0.53c | 0.79b | 1.08a | 0.064 | <0.001 | <0.001 | 0.303 | 0.123 |
表2 糖蜜添加量对杂交构树青贮营养成分含量的影响(干物质基础)
Table 2 Effects of addition amount of molasses on nutrient contents of hybrid B. papyrifera L. Vent silage(dry matter basis)
项目Item | 不同处理组Treatment | 标准误SEM | P值P value | ||||||
---|---|---|---|---|---|---|---|---|---|
CK | M5 | M10 | M20 | T | L | Q | C | ||
干物质DM/% | 24.41b | 26.83a | 28.02a | 28.32a | 0.466 | 0.001 | <0.001 | 0.027 | 0.898 |
粗蛋白质CP/% | 15.18b | 16.38ab | 16.98ab | 17.64a | 0.360 | 0.047 | 0.020 | 0.889 | 0.282 |
中性洗涤纤维NDF/% | 37.56a | 35.42a | 32.13b | 31.39b | 0.718 | <0.001 | <0.001 | 0.034 | 0.237 |
酸性洗涤纤维ADF/% | 20.88a | 20.48a | 19.41a | 17.41b | 0.403 | <0.001 | <0.001 | 0.490 | 0.658 |
酸性洗涤木质素ADL/% | 5.97 | 6.40 | 5.35 | 4.64 | 0.294 | 0.161 | 0.149 | 0.612 | 0.321 |
粗灰分Ash/% | 12.63a | 11.80b | 11.78b | 11.53b | 0.139 | 0.009 | 0.004 | 0.078 | 0.217 |
粗脂肪EE/% | 6.96 | 7.98 | 7.67 | 7.58 | 0.367 | 0.828 | 0.733 | 0.510 | 0.592 |
水溶性碳水化合物WSC/% | 0.54c | 0.53c | 0.79b | 1.08a | 0.064 | <0.001 | <0.001 | 0.303 | 0.123 |
项目Item | 不同处理组Treatments | 标准差 SEM | P值P value | ||||||
---|---|---|---|---|---|---|---|---|---|
CK | M5 | M10 | M20 | T | L | Q | C | ||
pH | 5.25a | 4.66b | 4.24c | 4.08d | 0.118 | <0.001 | <0.001 | <0.001 | 0.776 |
乳酸Lactic acid /(g·kg-1) | 21.64b | 46.98ab | 59.64a | 73.67a | 6.774 | 0.024 | 0.002 | 0.120 | 0.611 |
乙酸Acetic acid /(g·kg-1) | 24.96 | 26.04 | 24.09 | 22.72 | 0.649 | 0.342 | 0.131 | 0.595 | 0.401 |
丙酸Propionic acid /(g·kg-1) | 1.10a | 0.96ab | 0.52b | 0.44b | 0.104 | 0.043 | 0.010 | 0.380 | 0.358 |
丁酸Butyric acid /(g·kg-1) | 11.01a | 0.62b | 0.45b | 0.43b | 1.442 | 0.004 | 0.005 | 0.008 | 0.147 |
氨态氮/总氮NH3-N/TN /% | 13.40a | 7.02b | 5.18b | 2.53c | 1.092 | <0.001 | <0.001 | 0.003 | 0.150 |
表3 糖蜜添加量对杂交构树青贮发酵品质的影响(干物质基础)
Table 3 Effects of addition amount of molasses on the fermentation quality of hybrid B. papyrifera L. Vent silage(dry matter basis)
项目Item | 不同处理组Treatments | 标准差 SEM | P值P value | ||||||
---|---|---|---|---|---|---|---|---|---|
CK | M5 | M10 | M20 | T | L | Q | C | ||
pH | 5.25a | 4.66b | 4.24c | 4.08d | 0.118 | <0.001 | <0.001 | <0.001 | 0.776 |
乳酸Lactic acid /(g·kg-1) | 21.64b | 46.98ab | 59.64a | 73.67a | 6.774 | 0.024 | 0.002 | 0.120 | 0.611 |
乙酸Acetic acid /(g·kg-1) | 24.96 | 26.04 | 24.09 | 22.72 | 0.649 | 0.342 | 0.131 | 0.595 | 0.401 |
丙酸Propionic acid /(g·kg-1) | 1.10a | 0.96ab | 0.52b | 0.44b | 0.104 | 0.043 | 0.010 | 0.380 | 0.358 |
丁酸Butyric acid /(g·kg-1) | 11.01a | 0.62b | 0.45b | 0.43b | 1.442 | 0.004 | 0.005 | 0.008 | 0.147 |
氨态氮/总氮NH3-N/TN /% | 13.40a | 7.02b | 5.18b | 2.53c | 1.092 | <0.001 | <0.001 | 0.003 | 0.150 |
图1 杂交构树鲜样和青贮的Alpha多样性分析 各处理组标注不同字母表示具有显著差异(P < 0.05)。CK:对照组;M5:5 g/kg 糖蜜添加组;M10:10 g/kg 糖蜜添加组;M20:20 g/kg 糖蜜添加组,下同
Fig. 1 Alpha diversity analysis of hybrid B. papyrifera L. Vent fresh material and silage The histogram with different superscripts letters among treatments refer to significantly different(P < 0.05). CK:control group.M5:5 g/kg molasses group. M10:10 g/kg molasses group. M20:20 g/kg molasses group. The same below
图3 杂交构树鲜样和青贮在门水平(A)和属水平(B)的微生物群落组成
Fig. 3 Microbial community structure of hybrid B. papy-rifera L. Vent fresh material and silage at phylum(A)and genus(B)level
[1] | 陶莎, 张峭, 张晶. 2019年中国饲料市场回顾及2020年展望[J]. 农业展望, 2020, 16(4):12-17. |
Tao S, Zhang Q, Zhang J. China’s feed market situation in 2019 and outlook for 2020[J]. Agric Outlook, 2020, 16(4):12-17. | |
[2] | 邳植, 沈世华. 构树作为新兴的蛋白饲料原料的研究[J]. 饲料工业, 2018, 39(11):23-28. |
Pi Z, Shen SH. Research on paper mulberry as a new type of protein feedstuff[J]. Feed Ind, 2018, 39(11):23-28. | |
[3] | 屠焰, 刁其玉, 田莉, 等. 杂交构树营养成分瘤胃降解特点的研究[J]. 中国畜牧杂志, 2009, 45(11):38-41. |
Tu Y, Diao QY, Tian L, et al. Study on the rumen degradability of hybridized broussonetia papyrifera linn[J]. Chin J Animal Sci, 2009, 45(11):38-41. | |
[4] | 黄媛, 代胜, 等. 不同添加剂对构树青贮饲料发酵品质及微生物多样性的影响[J]. 动物营养学报, 2021, 33(3):1607-1617. |
Huang Y, Dai S, et al. Effects of different additives on fermentation quality and microbial diversity of paper mulberry silage[J]. Chin J Animal Nutr, 2021, 33(3):1607-1617. | |
[5] |
Mordenti AL, Giaretta E, et al. A review regarding the use of molasses in animal nutrition[J]. Animals, 2021, 11(1):115.
doi: 10.3390/ani11010115 URL |
[6] |
Kyum MK, Rahman SME, Islam MR, et al. A study on the growth performance of crossbred growing bull calves fed by supplementing molasses with straw based diets and conventional concentrates[J]. J Biol Sci, 2002, 3(1):26-31.
doi: 10.3923/jbs.2003.26.31 URL |
[7] | 张娟利, 莫放, 姚刚, 等. 日粮添加甜菜糖蜜提高能量进食水平对新疆褐牛增重性能和瘤胃发酵的影响[J]. 中国畜牧杂志, 2021, 57(4):153-158. |
Zhang JL, Mo F, Yao G, et al. Effect of increasing dietary energy intake by beet molasses addition on growth performance and rumen fermentation in Xinjiang brown bulls[J]. Chin J Animal Sci, 2021, 57(4):153-158. | |
[8] | 黄秋连, 周昕, 王健, 等. 添加乳酸菌、糖蜜和无机酸对羊草青贮饲料发酵品质及体外干物质消失率的影响[J]. 动物营养学报, 2021, 33(1):420-427. |
Huang QL, Zhou X, Wang J, et al. Effects of lactic acid bacteria, molasses and inorganic acid supplementations on fermentation quality and in vitro dry matter disappearance rate of Leymus chinensis silage[J]. Chin J Animal Nutr, 2021, 33(1):420-427. | |
[9] | 付锦涛, 王学凯, 等. 添加乳酸菌和糖蜜对全株构树和稻草混合青贮的影响[J]. 草业学报, 2020, 29(4):121-128. |
Fu JT, Wang XK, et al. The effects of adding lactic acid bacteria and molasses on fermentation of Broussonetia papyrifera and rice straw mixed silage[J]. Acta Prataculturae Sin, 2020, 29(4):121-128. | |
[10] | 穆麟, 李顺, 等. 添加糖蜜、乳酸菌制剂对籽粒苋与稻秸混合青贮品质的影响[J]. 草地学报, 2019, 27(2):482-487. |
Mu L, Li S, et al. Effect of adding molasses or lactic acid bacteria on quality of mixed silage of amaranth and rice straws[J]. Acta Agrestia Sin, 2019, 27(2):482-487. | |
[11] | AOAC. Official methods of analysis of AOAC[S]. 18th ed. Association of Analytical Chemists, Arlington, VA, 2005. |
[12] |
van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition[J]. J Dairy Sci, 1991, 74(10):3583-3597.
pmid: 1660498 |
[13] |
DuBois M, Gilles KA, Hamilton JK, et al. Colorimetric method for determination of sugars and related substances[J]. Anal Chem, 1956, 28(3):350-356.
doi: 10.1021/ac60111a017 URL |
[14] |
Wang J, Chen L, Yuan XJ, et al. Effects of molasses on the fermentation characteristics of mixed silage prepared with rice straw, local vegetable by-products and alfalfa in Southeast China[J]. J Integr Agric, 2017, 16(3):664-670.
doi: 10.1016/S2095-3119(16)61473-9 URL |
[15] |
Weatherburn MW. Phenol-hypochlorite reaction for determination of ammonia[J]. Anal Chem, 1967, 39(8):971-974.
doi: 10.1021/ac60252a045 URL |
[16] | 王亚芳, 姜富贵, 成海建, 等. 不同青贮添加剂对全株玉米青贮营养价值、发酵品质和瘤胃降解率的影响[J]. 动物营养学报, 2020, 32(6):2765-2774. |
Wang YF, Jiang FG, Cheng HJ, et al. Effects of different silage additives on nutritional value, fermentation quality and rumen degradability of whole corn silage[J]. Chin J Animal Nutr, 2020, 32(6):2765-2774. | |
[17] | 周昕, 黄秋连, 等. 添加乳酸菌剂和糖蜜对不同含水量食叶草青贮发酵品质及体外干物质消失率的影响[J]. 动物营养学报, 2021, 33(3):1594-1606. |
Zhou X, Huang QL, et al. Effects of adding lactic acid bacteria and molasses on fermentation quality and in vitro dry matter disappearance rate of Rumex hanus by. Silage with different moisture contents[J]. Chin J Animal Nutr, 2021, 33(3):1594-1606. | |
[18] |
Dunière L, Sindou J, Chaucheyras-Durand F, et al. Silage processing and strategies to prevent persistence of undesirable microorganisms[J]. Animal Feed Sci Technol, 2013, 182(1/2/3/4):1-15.
doi: 10.1016/j.anifeedsci.2013.04.006 URL |
[19] | 司丙文, 徐文财, 等. 不同添加剂对杂交构树青贮发酵品质的影响[J]. 动物营养学报, 2018, 30(11):4670-4675. |
Si BW, Xu WC, et al. Influence of different additives on fermentation quality of hybrid paper mulberry silage[J]. Chin J Animal Nutr, 2018, 30(11):4670-4675. | |
[20] | 韩哲宇, 毕小兵, 史海涛, 等. 构树青贮饲料开发利用技术研究进展[J]. 动物营养学报, 2021, 33(9):1-11. |
Han ZY, Bi XB, Shi HT, et al. Research advances in exploitation and utilization technology of broussonetia papyrifera silage[J]. Chin J Animal Nutr, 2021, 33(9):1-11. | |
[21] | 曹力凡, 高正龙, 陈谭星, 等. 不同收割高度和留茬高度对杂交构树产量、营养成分及消化率的影响[J]. 饲料研究, 2021, 44(5):88-91. |
Cao LF, Gao ZL, Chen TX, et al. Effect of different harvesting height and stubble height on yield, nutrient composition and digestibility of hybrid Broussonetia papyrifera[J]. Feed Res, 2021, 44(5):88-91. | |
[22] |
Jiang FG, Cheng HJ, Liu D, et al. Treatment of whole-plant corn silage with lactic acid bacteria and organic acid enhances quality by elevating acid content, reducing pH, and inhibiting undesirable microorganisms[J]. Front Microbiol, 2020, 11:593088.
doi: 10.3389/fmicb.2020.593088 URL |
[23] |
Wang YX, McAllister TA, et al. Effects of proanthocyanidins, dehulling and removal of pericarp on digestion of barley grain by ruminal micro-organisms[J]. J Sci Food Agric, 1999, 79(6):929-938.
doi: 10.1002/(ISSN)1097-0010 URL |
[24] | Woolford MK, Pahlow G. The silage fermentation[M]// Microbiology of Fermented Foods. Boston, MA:Springer US, 1998:73-102. |
[25] | 施巧婷, 姜富贵, 等. 氯化铵对全株玉米青贮营养价值和发酵品质的影响[J]. 动物营养学报, 2021, 33(4):2063-2072. |
Shi QT, Jiang FG, et al. Effects of ammonium chloride on nutritional value and fermentation quality of whole-plant corn silage[J]. Chin J Animal Nutr, 2021, 33(4):2063-2072. | |
[26] | Lindgren SE, Axelsson LT, McFeeters RF. Anaerobic l-lactate degradation by Lactobacillus plantarum[J]. FEMS Microbiol Lett, 1990, 66(1/2/3):209-213. |
[27] |
der Bedrosian MC, Nestor KE Jr, Kung L Jr. The effects of hybrid, maturity, and length of storage on the composition and nutritive value of corn silage[J]. J Dairy Sci, 2012, 95(9):5115-5126.
doi: S0022-0302(12)00527-9 pmid: 22916917 |
[28] | 李旭娇. 紫花苜蓿青贮饲料蛋白降解机制与调控研究[D]. 北京:中国农业大学, 2018. |
Li XJ. Research on mechanism and modification of protein degradation in alfalfa silage[D]. Beijing:China Agricultural University, 2018. | |
[29] |
Kunji ERS, Mierau I, Hagting A, et al. The proteotytic systems of lactic acid bacteria[J]. Antonie Van Leeuwenhoek, 1996, 70(2/3/4):187-221.
doi: 10.1007/BF00395933 URL |
[30] |
Ni K, Wang F, et al. Effects of lactic acid bacteria and molasses additives on the microbial community and fermentation quality of soybean silage[J]. Bioresour Technol, 2017, 238:706-715.
doi: 10.1016/j.biortech.2017.04.055 URL |
[31] |
Wang Y, Wang C, Zhou W, et al. Effects of wilting and Lactoba-cillus plantarum addition on the fermentation quality and microbial community of Moringa oleifera leaf silage[J]. Front Microbiol, 2018, 9:1817.
doi: 10.3389/fmicb.2018.01817 pmid: 30127780 |
[32] |
Guan H, Yan Y, Li X, et al. Microbial communities and natural fermentation of corn silages prepared with farm bunker-Silo in Southwest China[J]. Bioresour Technol, 2018, 265:282-290.
doi: 10.1016/j.biortech.2018.06.018 URL |
[33] |
Xu D, Ding W, Ke W, et al. Modulation of metabolome and bacterial community in whole crop corn silage by inoculating homofermentative Lactobacillus plantarum and heterofermentative Lactobacillus buchneri[J]. Front Microbiol, 2018, 9:3299.
doi: 10.3389/fmicb.2018.03299 URL |
[34] |
Ren F, He R, Zhou X, et al. Dynamic changes in fermentation profiles and bacterial community composition during sugarcane top silage fermentation:a preliminary study[J]. Bioresour Technol, 2019, 285:121315.
doi: 10.1016/j.biortech.2019.121315 URL |
[35] | 杨杨, 石超, 郭旭生. 高寒草甸魏斯氏乳酸菌的分离鉴定及理化特性研究[J]. 草业学报, 2014, 23(1):266-275. |
Yang Y, Shi C, Guo XS. Characterization and identification of Weissella species isolated from Kobresia littledalei growing in alpine meadows[J]. Acta Prataculturae Sin, 2014, 23(1):266-275. |
[1] | 梁成刚, 汪燕, 李天, 大杉立, 青木直大. SP1调控碳水化合物分配对穗形态的影响[J]. 生物技术通报, 2023, 39(5): 152-159. |
[2] | 任海伟, 孙一帆, 任雨薇, 郭晓鹏, 潘立超, 张丙云, 李金平. 基于文献计量的青贮添加剂研究进展[J]. 生物技术通报, 2022, 38(8): 261-274. |
[3] | 颜珲璘, 芦光新, 邓晔, 顾松松, 颜程良, 马坤, 赵阳安, 张海娟, 王英成, 周学丽, 窦声云. 高寒地区根瘤菌拌种对禾/豆混播土壤微生物群落的影响[J]. 生物技术通报, 2022, 38(10): 204-215. |
[4] | 陈梦言, 白洁, 柯文灿, 许冬梅, 艾琳, 郭旭生. 青贮饲料微生物群落组成与功能研究进展[J]. 生物技术通报, 2021, 37(9): 11-23. |
[5] | 辛亚芬, 陈晨, 曾泰儒, 杜昭昌, 倪浩然, 钟怡豪, 谭小平, 闫艳红. 青贮添加剂对微生物多样性影响的研究进展[J]. 生物技术通报, 2021, 37(9): 24-30. |
[6] | 王婷, 杨阳, 李金萍, 杜坤. 转基因作物对土壤微生物群落影响的研究进展[J]. 生物技术通报, 2021, 37(9): 255-265. |
[7] | 田静, 张建国. 植物表面乳酸菌分布研究进展[J]. 生物技术通报, 2021, 37(9): 3-10. |
[8] | 徐进益, 那彬彬, 刘顺, 陈超, 孙红, 郑玉龙. 青贮饲料的优良乳酸菌及其应用[J]. 生物技术通报, 2021, 37(9): 39-47. |
[9] | 张颖超, 尹守亮, 王一炜, 王学凯, 杨富裕. 木本饲料青贮研究进展[J]. 生物技术通报, 2021, 37(9): 48-57. |
[10] | 崔欣雨, 李荣荣, 蔡瑞, 王妍, 郑猛虎, 徐春城. 苜蓿青贮中乳酸降解菌的分离、鉴定及降解性能研究[J]. 生物技术通报, 2021, 37(9): 58-67. |
[11] | 王琦, 武之绚, 陈钟玲, 吴白乙拉, 胡宗福, 牛化欣. 副干酪乳杆菌对青贮苜蓿有氧暴露品质和细菌多样性的影响[J]. 生物技术通报, 2021, 37(9): 77-85. |
[12] | 毛婷, 牛永艳, 郑群, 杨涛, 穆永松, 祝英, 季彬, 王治业. 菌剂对苜蓿青贮发酵品质及微生物群落的影响[J]. 生物技术通报, 2021, 37(9): 86-94. |
[13] | 赵雅茹, 许庆方, 高文俊, 郭刚, 陈雷, 玉柱. 抑霉乳酸菌脱毒特性及青贮应用的研究[J]. 生物技术通报, 2021, 37(9): 95-105. |
[14] | 迪力热巴·阿不都肉苏力, 穆耶赛尔·奥斯曼, 祖力胡玛尔·肉孜, 马勤, 雷瑞峰, 安登第. 盐碱土壤微生物多样性与生物改良研究进展[J]. 生物技术通报, 2021, 37(10): 225-233. |
[15] | 黄婷, 方源, 冯舟, 沈和, 聂勇, 郑鑫, 汪家权, 许子牧. 高通量测序技术解析中学校园细菌群落的特征组成[J]. 生物技术通报, 2020, 36(8): 96-103. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||