ABA和干旱胁迫下菊花脑ZF-HD基因家族的表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2023-0530

摘要/Abstract

摘要：

干旱是影响菊花生长发育和产量的重要非生物因子，在应对干旱胁迫时ZF-HD转录因子家族起着重要的作用。菊花脑是研究菊花遗传育种机制的模式植物，筛选其抗旱相关基因可为菊花相关研究奠定基础。本研究在菊花脑基因组基础上，建立了本地数据库以实现本地BLAST，并利用Pfam数据库、HMMER、DNAMAN、MEGA、Tbtools、ExPASy、WOLF PSORT在线网站及实时荧光定量技术，对ZF-HD基因家族进行筛选以及生物信息学和表达分析。最终在菊花脑基因组中共鉴定出19个CnZF-HD基因，分为ZHD、MIF两个亚家族，其中ZHD亚族包括I-VI亚群。家族成员的组织特异性表达具有时间和空间差异性，且启动子区富含与生长发育、胁迫和激素相关的响应元件。RT-qPCR结果显示，19个CnZF-HD基因均响应干旱胁迫和ABA处理，且在干旱条件下均被诱导表达。CnZF-HD4、CnZF-HD5、CnZF-HD9、CnZF-HD10、CnZF-HD14、CnZF-HD15、CnZF-HD19这7个基因在ABA处理中被诱导表达，亚群VI在调控菊花脑抗旱性方面起着重要作用。另外，CnZF-HD1基因的表达在干旱条件下持续上调，可作为抗旱相关的候选基因，但其对ABA的敏感性需要进一步研究。

关键词: 菊花脑, 干旱胁迫, ZF-HD, 转录因子, ABA, 生物信息学, 表达分析

Abstract:

Drought is an important abiotic factor affecting the growth and yield of chrysanthemum, while ZF-HD transcription factor family plays an important role in coping with drought stress. Chrysanthemum nankingense is a model plant for studying genetic and breeding mechanism of chrysanthemum, screening its drought resistance related genes may lay a foundation for the relevant research in chrysanthemum. In this study, we established a local database and achieving local BLAST based on the C. nankingense genome, and used the Pfam database, HMMER, DNAMAN, MEGA, Tbtools, ExPASy, WoLF PSORT online sites and real-time fluorescence quantification for screening as well as bioinformatics and expression analysis of ZF-HD family genes. The results showed that a total of 19 ZF-HD gene family members were identified in the C. nankingense, divided into two subfamilies, ZHD and MIF, with the ZHD subfamily including subgroup I-VI. The tissue specific expressions of family members were temporally and spatially variable, their promoter regions were enriched with growth and development, stress and hormone-related response elements. The results of RT-qPCR showed that 19 CnZF-HD genes all responded to the drought stress and ABA treatment, and expression was induced under drought conditions. The genes CnZF-HD4, CnZF-HD9, CnZF-HD10, CnZF-HD14, CnZF-HD15 and CnZF-HD19 were also induced to be expressed in ABA treatment. Subgroup VI played an important role in regulating the drought resistance of C. nankingense. In addition, the expression of CnZF-HD1 gene was continuously up-regulated under drought conditions, thus it could be a candidate gene for drought resistance, but its sensitivity to ABA needs further study.

Key words: Chrysanthemum nankingense, drought stress, ZF-HD gene, transcription factor, ABA, bioinformatics, expression analysis

陈楚怡, 杨小梅, 陈胜艳, 陈斌, 岳莉然. ABA和干旱胁迫下菊花脑ZF-HD基因家族的表达分析[J]. 生物技术通报, 2023, 39(11): 270-282.

CHEN Chu-yi, YANG Xiao-mei, CHEN Sheng-yan, CHEN Bin, YUE Li-ran. Expression Analysis of the ZF-HD Gene Family in Chrysanthemum nankingense Under Drought and ABA Treatment[J]. Biotechnology Bulletin, 2023, 39(11): 270-282.

图/表 8

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基因 Gene	上游引物Forward primer（5'-3'）	下游引物Reverse primer（5'-3'）
CmEF1α	TTTTGGTATCTGGTCCTGGAG	CCATTCAAGCGACAGACTCA
CnZF-HD1	CCGCGTGTAACTGTCATCGT	GTGACGTGGATGGTAACGCT
CnZF-HD2	GTCACCACCATAGCAACTACCA	CCACCATAACCAGCACCATCT
CnZF-HD3	GAAGAGAAGAACCACCACCCT	AGTTTCCACCACCCTTCTATGG
CnZF-HD4	ACCACCTTACACCGCCTTTC	AAAGGACACGGGTAGTCCAC
CnZF-HD5	ATGCAGTAGACGGATGCAGAG	CTCTACGATGGAAATTCCTGTGGC
CnZF-HD6	CCCTCACCTTCAGCGTCATT	TTCTCCTTTTGTTCGGGCGT
CnZF-HD7	AGTTTCCACCGGAGAGAGGT	CATTTGCCCCACCAAATGCC
CnZF-HD8	TGGAGAAGAAGGTACACCCGA	TGATGGCTGATGCTCGGTTT
CnZF-HD9	TGGCCTCACTACCATCAACC	CAGTGTGTAGCGGCATGAAC
CnZF-HD10	CTTTGGATGGGTGTAGGGAGTT	TCCTCCTGTGGAAACTCTGGTG
CnZF-HD11	TACTACCCTTCAGCCCCACA	CATCTTCCATCCCACACGCT
CnZF-HD12	CCTTCAGCATTTGTGCCAGT	CATCCGGTCCTTCTGAGCTTT
CnZF-HD13	CTTCTGTAAATGCCCCACCC	TTCTCTTGGTATCCTCCACCC
CnZF-HD14	CACCAACCATTGCTACCTCCT	AACTCCCCGCAACCATCAAG
CnZF-HD15	CCACCCCATTCCCAATCAATC	TTTGAATGAAGATGGCTCCGCC
CnZF-HD16	TGGCTGTCACCGGAGTTTTC	ATCACCGGCACCGTTTTTGG
CnZF-HD17	TCCACCATGCAAGTGTACC	TCCCTCTTCTTATCCCAATCCC
CnZF-HD18	TGGGAGACGAGAAAAAGAGTCC	CACCTTTAGTGCCCTCCTC
CnZF-HD19	TGGTTGCGGGGAGTTTATGT	TGTGAGGTGGTGGTGGATAG

基因 Gene	上游引物Forward primer（5'-3'）	下游引物Reverse primer（5'-3'）
CmEF1α	TTTTGGTATCTGGTCCTGGAG	CCATTCAAGCGACAGACTCA
CnZF-HD1	CCGCGTGTAACTGTCATCGT	GTGACGTGGATGGTAACGCT
CnZF-HD2	GTCACCACCATAGCAACTACCA	CCACCATAACCAGCACCATCT
CnZF-HD3	GAAGAGAAGAACCACCACCCT	AGTTTCCACCACCCTTCTATGG
CnZF-HD4	ACCACCTTACACCGCCTTTC	AAAGGACACGGGTAGTCCAC
CnZF-HD5	ATGCAGTAGACGGATGCAGAG	CTCTACGATGGAAATTCCTGTGGC
CnZF-HD6	CCCTCACCTTCAGCGTCATT	TTCTCCTTTTGTTCGGGCGT
CnZF-HD7	AGTTTCCACCGGAGAGAGGT	CATTTGCCCCACCAAATGCC
CnZF-HD8	TGGAGAAGAAGGTACACCCGA	TGATGGCTGATGCTCGGTTT
CnZF-HD9	TGGCCTCACTACCATCAACC	CAGTGTGTAGCGGCATGAAC
CnZF-HD10	CTTTGGATGGGTGTAGGGAGTT	TCCTCCTGTGGAAACTCTGGTG
CnZF-HD11	TACTACCCTTCAGCCCCACA	CATCTTCCATCCCACACGCT
CnZF-HD12	CCTTCAGCATTTGTGCCAGT	CATCCGGTCCTTCTGAGCTTT
CnZF-HD13	CTTCTGTAAATGCCCCACCC	TTCTCTTGGTATCCTCCACCC
CnZF-HD14	CACCAACCATTGCTACCTCCT	AACTCCCCGCAACCATCAAG
CnZF-HD15	CCACCCCATTCCCAATCAATC	TTTGAATGAAGATGGCTCCGCC
CnZF-HD16	TGGCTGTCACCGGAGTTTTC	ATCACCGGCACCGTTTTTGG
CnZF-HD17	TCCACCATGCAAGTGTACC	TCCCTCTTCTTATCCCAATCCC
CnZF-HD18	TGGGAGACGAGAAAAAGAGTCC	CACCTTTAGTGCCCTCCTC
CnZF-HD19	TGGTTGCGGGGAGTTTATGT	TGTGAGGTGGTGGTGGATAG

基因ID Gene ID	基因名称 Gene name	氨基酸 Number of amino acids/aa	分子量 Molecular weight/Da	等电点 pI	亚细胞定位 Predicted location	磷酸化位点数目Phosphorylation site
基因ID Gene ID	基因名称 Gene name	氨基酸 Number of amino acids/aa	分子量 Molecular weight/Da	等电点 pI	亚细胞定位 Predicted location	丝氨酸 Ser	苏氨酸 Thr	酪氨酸 Tyr	总数 Total number
CHR00000129	CnZF-HD1	239	26 293.14	8.70	细胞核 Nucleus	16	12	1	29
CHR00004454	CnZF-HD2	324	36 580.21	7.14	细胞核 Nucleus	11	7	3	21
CHR00008752	CnZF-HD3	95	10 527.72	5.77	细胞质 Cytoplasm	7	4	1	12
CHR00009701	CnZF-HD4	337	37 541.18	6.80	细胞核 Nucleus	24	12	4	40
CHR00010377	CnZF-HD5	94	10 259.57	7.60	线粒体 Mitochondria	9	6	0	15
CHR00019161	CnZF-HD6	267	29 598.21	6.83	细胞核 Nucleus	15	15	4	34
CHR00030290	CnZF-HD7	434	49 475.83	9.57	叶绿体 Chloroplast	24	19	3	46
CHR00046942	CnZF-HD8	263	30 175.71	8.21	细胞核 Nucleus	15	11	3	29
CHR00047218	CnZF-HD9	258	28 269.12	9.27	细胞核 Nucleus	17	4	3	24
CHR00048774	CnZF-HD10	85	9 282.57	7.67	叶绿体和线粒体 Chloroplast and Mitochondria	5	1	1	7
CHR00051473	CnZF-HD11	349	38 055.30	7.79	细胞核 Nucleus	22	14	1	37
CHR00055582	CnZF-HD12	191	21 238.01	9.71	细胞核 Nucleus	12	5	1	18
CHR00058475	CnZF-HD13	289	32 048.70	8.49	细胞核 Nucleus	23	11	2	36
CHR00061161	CnZF-HD14	145	16 331.64	7.08	叶绿体 Chloroplast	14	3	0	17
CHR00077153	CnZF-HD15	218	23 736.40	6.19	细胞核 Nucleus	12	18	1	31
CHR00081707	CnZF-HD16	123	13 516.44	9.87	线粒体 Mitochondria	9	4	1	14
CHR00082507	CnZF-HD17	309	33 442.00	6.63	细胞核 Nucleus	17	24	2	43
CHR00084858	CnZF-HD18	91	10 311.30	4.69	细胞核 Nucleus	7	1	2	10
CHR00091694	CnZF-HD19	173	19 332.72	6.27	叶绿体 Chloroplast	16	7	1	24

基因ID Gene ID	基因名称 Gene name	氨基酸 Number of amino acids/aa	分子量 Molecular weight/Da	等电点 pI	亚细胞定位 Predicted location	磷酸化位点数目Phosphorylation site
基因ID Gene ID	基因名称 Gene name	氨基酸 Number of amino acids/aa	分子量 Molecular weight/Da	等电点 pI	亚细胞定位 Predicted location	丝氨酸 Ser	苏氨酸 Thr	酪氨酸 Tyr	总数 Total number
CHR00000129	CnZF-HD1	239	26 293.14	8.70	细胞核 Nucleus	16	12	1	29
CHR00004454	CnZF-HD2	324	36 580.21	7.14	细胞核 Nucleus	11	7	3	21
CHR00008752	CnZF-HD3	95	10 527.72	5.77	细胞质 Cytoplasm	7	4	1	12
CHR00009701	CnZF-HD4	337	37 541.18	6.80	细胞核 Nucleus	24	12	4	40
CHR00010377	CnZF-HD5	94	10 259.57	7.60	线粒体 Mitochondria	9	6	0	15
CHR00019161	CnZF-HD6	267	29 598.21	6.83	细胞核 Nucleus	15	15	4	34
CHR00030290	CnZF-HD7	434	49 475.83	9.57	叶绿体 Chloroplast	24	19	3	46
CHR00046942	CnZF-HD8	263	30 175.71	8.21	细胞核 Nucleus	15	11	3	29
CHR00047218	CnZF-HD9	258	28 269.12	9.27	细胞核 Nucleus	17	4	3	24
CHR00048774	CnZF-HD10	85	9 282.57	7.67	叶绿体和线粒体 Chloroplast and Mitochondria	5	1	1	7
CHR00051473	CnZF-HD11	349	38 055.30	7.79	细胞核 Nucleus	22	14	1	37
CHR00055582	CnZF-HD12	191	21 238.01	9.71	细胞核 Nucleus	12	5	1	18
CHR00058475	CnZF-HD13	289	32 048.70	8.49	细胞核 Nucleus	23	11	2	36
CHR00061161	CnZF-HD14	145	16 331.64	7.08	叶绿体 Chloroplast	14	3	0	17
CHR00077153	CnZF-HD15	218	23 736.40	6.19	细胞核 Nucleus	12	18	1	31
CHR00081707	CnZF-HD16	123	13 516.44	9.87	线粒体 Mitochondria	9	4	1	14
CHR00082507	CnZF-HD17	309	33 442.00	6.63	细胞核 Nucleus	17	24	2	43
CHR00084858	CnZF-HD18	91	10 311.30	4.69	细胞核 Nucleus	7	1	2	10
CHR00091694	CnZF-HD19	173	19 332.72	6.27	叶绿体 Chloroplast	16	7	1	24

基因 Gene	处理时间 Treatment time/h
基因 Gene	0	2	6	12	24
CnZF-HD1	（1.00±0.00）d	（1.52±0.11）c	（4.02±0.03）b	（4.36±0.02）b	（5.14±0.05）a
CnZF-HD2	（1.00±0.00）d	（1.76±0.13）c	（3.05±0.02）a	（2.15±0.04）b	（1.53±0.04）c
CnZF-HD3	（1.00±0.00）b	（2.12±0.03）a	（1.34±0.07）b	（1.24±0.05）b	（1.13±0.04）b
CnZF-HD4	（1.00±0.00）b	（1.05±0.02）b	（1.87±0.11）a	（0.84±0.09）c	（0.73±0.11）c
CnZF-HD5	（1.00±0.00）d	（1.48±0.11）c	（3.41±0.06）b	（3.97±0.07）a	（1.41±0.09）c
CnZF-HD6	（1.00±0.00）c	（1.28±0.03）b	（1.38±0.10）b	（2.13±0.05）a	（0.94±0.07）c
CnZF-HD7	（1.00±0.00）c	（2.14±0.14）a	（1.35±0.12）b	（1.19±0.11）c	（0.58±0.08）d
CnZF-HD8	（1.00±0.00）c	（2.08±0.09）a	（1.28±0.08）b	（1.2±0.06）b	（0.62±0.07）d
CnZF-HD9	（1.00±0.00）c	（1.32±0.09）b	（1.56±0.07）a	（0.84±0.11）d	（0.71±0.12）d
CnZF-HD10	（1.00±0.00）c	（1.88±0.06）a	（1.34±0.12）b	（1.21±0.13）b	（1.08±0.12）c
CnZF-HD11	（1.00±0.00）c	（1.21±0.05）b	（1.53±0.04）a	（1.37±0.14）b	（1.14±0.11）c
CnZF-HD12	（1.00±0.00）c	（1.28±0.05）b	（2.19±0.09）a	（0.78±0.06）d	（0.68±0.07）d
CnZF-HD13	（1.00±0.00）c	（1.24±0.04）b	（1.29±0.14）b	（1.75±0.03）a	（0.63±0.02）d
CnZF-HD14	（1.00±0.00）c	（1.89±0.05）b	（2.17±0.10）a	（0.81±0.11）b	（0.73±0.13）d
CnZF-HD15	（1.00±0.00）d	（1.52±0.13）c	（3.49±0.07）b	（4.08±0.08）a	（1.39±0.08）c
CnZF-HD16	（1.00±0.00）c	（1.53±0.13）b	（3.53±0.08）a	（3.78±0.11）a	（1.43±0.11）b
CnZF-HD17	（1.00±0.00）b	（1.12±0.11）b	（1.38±0.05）a	（1.26±0.04）a	（0.72±0.07）c
CnZF-HD18	（1.00±0.00）c	（1.32±0.06）b	（2.04±0.07）a	（1.08±0.11）c	（0.74±0.09）d
CnZF-HD19	（1.00±0.00）b	（2.01±0.07）a	（2.17±0.08）a	（0.83±0.08）c	（0.74±0.06）c