一株高产吲哚乙酸的Bacillus cereus YT2-1C的鉴定及促生作用

doi:10.13560/j.cnki.biotech.bull.1985.2024-1076

摘要/Abstract

摘要：

目的分析高产吲哚乙酸的YT2-1C菌株促生抑菌潜力，为其后续开发利用提供基础。方法通过菌株形态特征、16S rRNA和31个看家基因比对结果对菌株进行鉴定，定量测定菌株IAA的产量，分析菌株在产铁载体、溶磷、解钾、产蛋白酶、固氮和抑菌方面的潜力。进一步通过育苗和田间试验验证菌株的促生效果，并从基因组水平初步分析了菌株与促生相关的功能基因。结果 YT2-1C菌株鉴定为蜡样芽胞杆菌（Bacillus cereus），该菌株IAA产量高达193 μg/mL，具有产铁载体、溶磷、产蛋白酶和抑菌的能力。育苗试验结果表明，接种YT2-1C菌株的基质，显著增加黄瓜、辣椒和番茄的株高、叶绿素、茎粗和鲜重；黄瓜移栽田间后，仍有较好的促生作用。全基因组测序分析表明YT2-1C菌株具有54个与促生相关的基因，5个非核糖体肽合成相关的基因。结论蜡样芽胞杆菌YT2-1C是一株具有促生防病功能的菌株，对黄瓜、辣椒和番茄有显著的促生作用，含有多个与促生防病相关基因，为其微生物菌剂的研制奠定了基础。

关键词: 促生菌, 蜡样芽胞杆菌, 菌株YT2-1C, IAA, 育苗基质, 全基因组测序

Abstract:

Objective The growth promotion and bacteriostatic potential of strain YT2-1C with high yield of indoleacetic acid was analyzed to provide a basis for its subsequent development and utilization. Method The strain was identified by morphological characteristics, 16S rRNA and 31 house-keeping genes. The IAA yield of strain was quantitatively determined. The potential of the strain in iron production, phosphorus solubilization, potassium solubilization, protease production, nitrogen fixation and bacteriostasis were analyzed. The growth-promoting effect of the strain was further verified by seedling breeding and field experiments. The functional genes related to growth-promoting were preliminarily analyzed from the genome level. Result The strain was identified as Bacillus cereus. The IAA yield of this strain reached 193 μg/ml. It had the ability of producing iron carrier, dissolving phosphorus, producing protease, and inhibiting bacteria. The results from breeding experiment showed that the plant height, chlorophyll, stem diameter and fresh weight of cucumber, pepper and tomato significantly increased by inoculation of YT2-1C strain. There was still good growth-promoting effect after cucumber plants transplanted to the field. Whole genome sequencing analysis showed that strain YT2-1C had 54 genes related to growth promotion and 5 genes related to non-ribosomal peptide synthesis. Conclusion Bacillus cereus YT2-1C is a strain that has the function of promoting growth and preventing disease. It has significant growth-promoting effect on cucumber, pepper and tomato, and contains many genes related to growth-promoting and disease-preventing, which lays a foundation for the development of its microbial microbial agent.

Key words: growth-promoting bacteria, Bacillus cereus, strain YT2-1C, IAA, seedling substrate, whole genome sequencing

张慧, 卢文才, 王冬, 刘倩, 马连杰. 一株高产吲哚乙酸的Bacillus cereus YT2-1C的鉴定及促生作用[J]. 生物技术通报, 2025, 41(5): 300-309.

ZHANG Hui, LU Wen-cai, WANG Dong, LIU Qian, MA Lian-jie. Identification of Bacillus cereus YT2-1C with High Indoleacetic Acid Yield and Its Growth-promoting Effect[J]. Biotechnology Bulletin, 2025, 41(5): 300-309.

图/表 10

参考文献 37

1	马雪晴, 冀傲冉, 郑娇莉, 等. 植物根际促生菌促生机制及其应用研究进展 [J]. 中国农业科技导报, 2025, 27(2): 13-23.
	Ma XQ, Ji AR, Zheng JL, et al. Research progress on growth promotion mechanism and its application in plant growth promoting rhizobacteria [J]. China Ind Econ, 2025, 27(2): 13-23.
2	徐明岗, 卢昌艾, 张文菊, 等. 我国耕地质量状况与提升对策 [J]. 中国农业资源与区划, 2016, 37(7): 8-14.
	Xu MG, Lu CA, Zhang WJ, et al. Situation of the quality of arable land in China and improvement strategy [J]. Chin J Agric Resour Reg Plan, 2016, 37(7): 8-14.
3	沈仁芳, 王超, 孙波."藏粮于地、藏粮于技"战略实施中的土壤科学与技术问题 [J]. 中国科学院院刊, 2018, 33(2): 135-144.
	Shen RF, Wang C, Sun B. Soil related scientific and technological problems in implementing strategy of "storing grain in land and technology" [J]. Bull Chin Acad Sci, 2018, 33(2): 135-144.
4	徐明岗, 段英华, 白珊珊, 等. 基于长期定位试验的土壤健康研究与展望 [J]. 植物营养与肥料学报, 2024, 30(7): 1253-1261.
	Xu MG, Duan YH, Bai SS, et al. Research and prospects for soil health based on long-term experiments in arable land of China [J]. Plant Nutr Fert Sci, 2024, 30(7): 1253-1261.
5	Vejan P, Abdullah R, Khadiran T, et al. Role of plant growth promoting rhizobacteria in agricultural sustainability-a review [J]. Molecules, 2016, 21(5): 573.
6	周益帆, 王金斌, 何川, 等. 一株产吲哚乙酸的Bacillus velezensis JB0319的筛选、鉴定及其促生作用 [J]. 土壤通报, 2024, 55(1): 173-183.
	Zhou YF, Wang JB, He C, et al. Screening, identification and growth promotion of A strain of Bacillus velezensis JB0319 producing indoleacetic acid [J]. Chin J Soil Sci, 2024, 55(1): 173-183.
7	徐科玉. 高产吲哚乙酸微生物菌株的筛选、发酵及其促生效果 [D]. 石家庄: 河北科技大学, 2022.
	Xu KY. Screening, fermentation and growth-promoting effect of microbial strains with high indole acetic acid production [D]. Shijiazhuang: Hebei University of Science and Technology, 2022.
8	da Silva MJC, Junior SFP, Junior KF, et al. IAA production of indigenous isolate of plant growth promoting rhizobacteria in the presence of tryptophan [J]. Aust J Crop Sci, 2020(14(03): 2020): 537-544.
9	de Garcia Salamone IE, Hynes RK, Nelson LM. Role of cytokinins in plant growth promotion by rhizosphere bacteria [M]//PGPR: Biocontrol and Biofertilization. Dordrecht: Springer Netherlands, 2005: 173-195.
10	James EK, Gyaneshwar P, Mathan N, et al. Infection and colonization of rice seedlings by the plant growth-promoting bacterium Herbaspirillum seropedicae Z67 [J]. Mol Plant Microbe Interact, 2002, 15(9): 894-906.
11	万雨欣, 徐伟慧, 胡云龙, 等. 玉米根际促生菌的筛选鉴定及促生效果评价 [J]. 玉米科学, 2024, 32(2): 144-153.
	Wan YX, Xu WH, Hu YL, et al. Screening and identification of maize rhizosphere growth-promoting bacteria and evaluation of their growth-promoting effects [J]. J Maize Sci, 2024, 32(2): 144-153.
12	陈伟, 舒健虹, 陈莹, 等. 黑麦草根际铁载体产生菌WN-H3的分离鉴定及其产铁载体培养条件的优化 [J]. 生物技术通报, 2016, 32(10): 219-226.
	Chen W, Shu JH, Chen Y, et al. Screening, identification and fermentation condition optimun of a siderophore-producing bacteria WN-H3 from rhizosphere of ryegrass [J]. Biotechnol Bull, 2016, 32(10): 219-226.
13	郇惠杰, 钟泓波, 雷芬芬, 等. 产蛋白酶海洋细菌的筛选、鉴定及发酵培养基的研究 [J]. 食品工业科技, 2013, 34(24): 181-185.
	Huan HJ, Zhong HB, Lei FF, et al. Study on isolation and identification of protease-producing marine bacteria and optimization of fermentation medium [J]. Sci Technol Food Ind, 2013, 34(24): 181-185.
14	张祥胜. 发酵液有效磷含量测定方法研究 [J]. 湖州职业技术学院学报, 2008, 6(3): 1-3.
	Zhang XS. A study of factors affecting the determined value by Mo-Sn-vc method of organic phosphobacteria [J]. J Huzhou Vocat Technol Coll, 2008, 6(3): 1-3.
15	李浩. 玉米、黄瓜根际促生菌组合优化及基因组测序 [D]. 泰安: 山东农业大学, 2019.
	Li H. Optimization of rhizosphere growth-promoting bacteria combination and genome sequencing of maize and cucumber [D]. Taian: Shandong Agricultural University, 2019.
16	张垚, 张芝, 王志刚, 等. 辣椒根际促生菌筛选鉴定及其促生效应初探 [J]. 浙江农业科学, 2022, 63(5): 958-963.
	Zhang Y, Zhang Z, Wang ZG, et al. J Zhejiang Agric Sci, 2022, 63(5): 958-963.
17	伍巧慧, 龚文坤, 刘新月, 等. 哈密瓜根际耐高温促生菌的筛选及其促生效应研究 [J]. 中国土壤与肥料, 2023(11): 221-228.
	Wu QH, Gong WK, Liu XY, et al. Screening of high temperature resistant growth promoting bacteria in the rhizosphere of cantaloupe and its promotion effect [J]. Soils Fertil Sci China, 2023(11): 221-228.
18	葛淼淼, 薄永琳, 刘宸, 等. 土壤产铁载体细菌的筛选及其对铁氧化物的活化与利用 [J]. 微生物学通报, 2023, 50(3): 1062-1072.
	Ge MM, Bo YL, Liu C, et al. Screening of soil siderophore-producing bacteria and their activation and utilization of iron oxide [J]. Microbiol China, 2023, 50(3): 1062-1072.
19	谢东, 汪流伟, 李宁健, 等. 一株多功能菌株的发掘、鉴定及解磷条件优化 [J]. 生物技术通报, 2023, 39(7): 241-253.
	Xie D, Wang LW, Li NJ, et al. Exploration, identification and phosphorus-solubilizing condition optimization of a multifunctional strain [J]. Biotechnol Bull, 2023, 39(7): 241-253.
20	俞海平, 傅庆林, 刘俊丽, 等. 解钾细菌的分离筛选及其对水稻的促生效果 [J]. 浙江农业科学, 2022, 63(6): 1161-1164.
	Yu HP, Fu QL, Liu JL, et al. Isolation and identification of decomposing potassium bacteria and its promoting effect on rice [J]. J Zhejiang Agric Sci, 2022, 63(6): 1161-1164.
21	耿芳, 杨绍青, 闫巧娟, 等. 土壤中高产蛋白酶菌株的筛选鉴定及发酵条件优化 [J]. 中国酿造, 2018, 37(4): 66-71.
	Geng F, Yang SQ, Yan QJ, et al. Screening, identification and fermentation conditions optimization of a high yield protease strain from soil [J]. China Brew, 2018, 37(4): 66-71.
22	Ku YL, Xu GY, Tian XH, et al. Root colonization and growth promotion of soybean, wheat and Chinese cabbage by Bacillus cereus YL6 [J]. PLoS One, 2018, 13(11): e0200181.
23	Kumar P, Pahal V, Gupta A, et al. Effect of silver nanoparticles and Bacillus cereus LPR2 on the growth of Zea mays [J]. Sci Rep, 2020, 10(1): 20409.
24	Ali AM, Awad MYM, Hegab SA, et al. Effect of potassium solubilizing bacteria (Bacillus cereus) on growth and yield of potato [J]. J Plant Nutr, 2021, 44(3): 411-420.
25	Sherpa MT, Bag N, Das S, et al. Isolation and characterization of plant growth promoting rhizobacteria isolated from organically grown high yielding pole type native pea (Pisum sativum L.) variety Dentami of Sikkim, India [J]. Curr Res Microb Sci, 2021, 2: 100068.
26	Ibrahim MS, Ikhajiagbe B. The growth response of rice (Oryza sativa L. var. FARO 44) in vitro after inoculation with bacterial isolates from a typical ferruginous ultisol [J]. Bull Natl Res Cent, 2021, 45(1): 70.
27	Li JG, Jiang ZQ, Xu LP, et al. Characterization of chitinase secreted by Bacillus cereus strain CH2 and evaluation of its efficacy against Verticillium wilt of eggplant [J]. BioControl, 2008, 53(6): 931-944.
28	Baliyan N, Dhiman S, Dheeman S, et al. Optimization of gibberellic acid production in endophytic Bacillus cereus using response surface methodology and its use as plant growth regulator in chickpea [J]. J Plant Growth Regul, 2022, 41(7): 3019-3029.
29	Adeleke BS, Ayangbenro AS, Babalola OO. Genomic analysis of endophytic Bacillus cereus T4S and its plant growth-promoting traits [J]. Plants, 2021, 10(9): 1776.
30	Zhou H, Ren ZH, Zu X, et al. Efficacy of plant growth-promoting bacteria Bacillus cereus YN917 for biocontrol of rice blast [J]. Front Microbiol, 2021, 12: 684888.
31	Maheshwari DK. Bacterial metabolites in sustainable agroecosystem [M].Springer Cham, 2015.
32	王丽, 王剑峰, 孔鑫, 等. PGPR促进植物生长及增强植物耐镉机制研究进展 [J]. 种子, 2024, 43(6): 73-85.
	Wang L, Wang JF, Kong X, et al. Progress on the mechanism of PGPR to promote plant growth and enhance cadmium tolerance in plants [J]. Seed, 2024, 43(6): 73-85.
33	AHMED RAGAB HENAWY FARAG ELGABALY. 一种新型的可促进植物生长和固氮的微生物Burkholderia sp. HAN2018的基因组分析 [D]. 武汉: 华中农业大学, 2019.
	AHMEDRAGAB HENAWY FARAG ELGABALY. Genome analysis of Burkholderia sp. HAN 2018, a new microorganism that can promote plant growth and nitrogen fixation [D]. Wuhan: Huazhong Agricultural University, 2019.
34	Sharma A, Dev K, Sourirajan A, et al. Isolation and characterization of salt-tolerant bacteria with plant growth-promoting activities from saline agricultural fields of Haryana, India [J]. J Genet Eng Biotechnol, 2021, 19(1): 99.
35	曹倩荣, 刘春卯, 郑翔, 等. 蛋白酶枯草芽孢杆菌工程菌株WB800N/pHT43-npr-PrsA的发酵工艺优化 [J]. 饲料研究, 2024, 47(14): 100-106.
	Cao QR, Liu CM, Zheng X, et al. Optimization of fermentation process for Bacillus subtilis WB800N/pHT43-npr-PrsA recombinant strain with protease activity [J]. Feed Res, 2024, 47(14): 100-106.
36	Etesami H, Maheshwari DK. Use of plant growth promoting rhizobacteria (PGPRs) with multiple plant growth promoting traits in stress agriculture: Action mechanisms and future prospects [J]. Ecotoxicol Environ Saf, 2018, 156: 225-246.
37	Glazer AN, Kechris KJ. Conserved amino acid sequence features in the alpha subunits of MoFe, VFe, and FeFe nitrogenases [J]. PLoS One, 2009, 4(7): e6136.

作物 Crop	处理 Treatment	株高 Plant height （cm）	叶绿素Chlorophyll （SPAD）	茎粗 Stem size （mm）	鲜重 Fresh weight （g）
辣椒	CK	6.20a	33.28a	2.02a	0.86a
辣椒	YT2-1C	10.2b	37.28b	2.64b	1.33b
番茄	CK	8.32a	2.84a	2.02a	1.78a
番茄	YT2-1C	19.12b	5.21b	2.64b	7.49b

作物 Crop	处理 Treatment	株高 Plant height （cm）	叶绿素Chlorophyll （SPAD）	茎粗 Stem size （mm）	鲜重 Fresh weight （g）
辣椒	CK	6.20a	33.28a	2.02a	0.86a
辣椒	YT2-1C	10.2b	37.28b	2.64b	1.33b
番茄	CK	8.32a	2.84a	2.02a	1.78a
番茄	YT2-1C	19.12b	5.21b	2.64b	7.49b

功能 Function	基因 Gene	产物 Product
IAA合成 IAA synthesis	trpA	色氨酸合成酶ɑ亚基
	trpB	色氨酸合成酶β亚基
	trpC	吲哚-3-甘油磷酸合成酶
	trpD	邻氨基苯甲酸磷酸核糖基转移酶
	trpE	邻氨基苯甲酸合酶
	trpG	邻氨基苯甲酸合酶
	ipdC	吲哚3-丙酮酸脱羧酶
	aroA	3-磷酸莽草酸1-羧乙烯基转移酶
	sucA	2-氧戊二酸脱氢酶E1组分
	kynB	芳基甲酰胺酶
	kynU	尿氨酸酶
	atoB	乙酰辅酶a-c-乙酰转移酶
	amiE	酰胺酶家族
	aofH	黄素单胺氧化酶
铁载体 Siderophore	entA	2，3-二氢-2，3-二羟基苯甲酸脱氢酶
	entB	异分支酸酶家族蛋白
	entC	异氯酸合成酶DhbC
	entE	（2，3-二羟基苯甲酰）腺苷酸合酶
	menF	同氯酸酯合成酶
	mbtH	MbtH家族蛋白
	nuoA	NADH-醌氧化还原酶亚基NuoA
	nuoB	NADH-醌氧化还原酶亚基NuoB
	nuoC	NADH-醌氧化还原酶亚基C
	nuoD	NADH-醌氧化还原酶亚基NuoD
	nuoI	NADH-醌氧化还原酶亚基nui
	nuoJ	NADH-醌氧化还原酶亚基J
	nuoH	NADH-醌氧化还原酶亚基NuoH
	nuoK	NADH-醌氧化还原酶亚基NuoK
	nuoL	NADH-醌氧化还原酶亚基L
	nuoN	NADH-醌氧化还原酶亚基NuoN
	nuoM	NADH-醌氧化还原酶亚基M
	bfr	类铁蛋白结构域含蛋白
	ftnA	铁蛋白
溶磷 Phosphorus solubilization	patA	转氨酶A
	pstA	磷酸ABC转运蛋白渗透酶
	pstC	磷酸ABC转运蛋白渗透酶
	pyk	丙酮酸激酶
	pyc	丙酮酸羧化酶
	ackA	醋酸激酶
	gltA	柠檬酸合酶
	buk	丁酸激酶
	aroK	莽草激酶
产蛋白酶 Proteinase production	aprE	S8家族肽酶
	nprE	M4家族金属肽酶
	nprB	中性蛋白酶B前体
	vpr	S8家族丝氨酸肽酶
	mprF	双功能赖氨酸磷脂酰甘油翻转酶/合成酶
固氮 Nitrogen fixation	glnA	谷氨酸-氨连接酶
	gltB	谷氨酸合酶
	narG	硝酸盐还原酶
	narH	硝酸盐还原酶
	narI	亚硝酸盐氧化还原酶
	nirA	铁氧还蛋白-亚硝酸盐还原酶
	cynT	碳酸酐酶
非核糖体肽 Non-ribosomal peptides	tycC	非核糖体肽合成酶
	dhbF	非核糖体肽合成酶
	mcyA	非核糖体肽合成酶
	ituB	非核糖体肽合成酶
	bacA	非核糖体肽合成酶

功能 Function	基因 Gene	产物 Product
IAA合成 IAA synthesis	trpA	色氨酸合成酶ɑ亚基
	trpB	色氨酸合成酶β亚基
	trpC	吲哚-3-甘油磷酸合成酶
	trpD	邻氨基苯甲酸磷酸核糖基转移酶
	trpE	邻氨基苯甲酸合酶
	trpG	邻氨基苯甲酸合酶
	ipdC	吲哚3-丙酮酸脱羧酶
	aroA	3-磷酸莽草酸1-羧乙烯基转移酶
	sucA	2-氧戊二酸脱氢酶E1组分
	kynB	芳基甲酰胺酶
	kynU	尿氨酸酶
	atoB	乙酰辅酶a-c-乙酰转移酶
	amiE	酰胺酶家族
	aofH	黄素单胺氧化酶
铁载体 Siderophore	entA	2，3-二氢-2，3-二羟基苯甲酸脱氢酶
	entB	异分支酸酶家族蛋白
	entC	异氯酸合成酶DhbC
	entE	（2，3-二羟基苯甲酰）腺苷酸合酶
	menF	同氯酸酯合成酶
	mbtH	MbtH家族蛋白
	nuoA	NADH-醌氧化还原酶亚基NuoA
	nuoB	NADH-醌氧化还原酶亚基NuoB
	nuoC	NADH-醌氧化还原酶亚基C
	nuoD	NADH-醌氧化还原酶亚基NuoD
	nuoI	NADH-醌氧化还原酶亚基nui
	nuoJ	NADH-醌氧化还原酶亚基J
	nuoH	NADH-醌氧化还原酶亚基NuoH
	nuoK	NADH-醌氧化还原酶亚基NuoK
	nuoL	NADH-醌氧化还原酶亚基L
	nuoN	NADH-醌氧化还原酶亚基NuoN
	nuoM	NADH-醌氧化还原酶亚基M
	bfr	类铁蛋白结构域含蛋白
	ftnA	铁蛋白
溶磷 Phosphorus solubilization	patA	转氨酶A
	pstA	磷酸ABC转运蛋白渗透酶
	pstC	磷酸ABC转运蛋白渗透酶
	pyk	丙酮酸激酶
	pyc	丙酮酸羧化酶
	ackA	醋酸激酶
	gltA	柠檬酸合酶
	buk	丁酸激酶
	aroK	莽草激酶
产蛋白酶 Proteinase production	aprE	S8家族肽酶
	nprE	M4家族金属肽酶
	nprB	中性蛋白酶B前体
	vpr	S8家族丝氨酸肽酶
	mprF	双功能赖氨酸磷脂酰甘油翻转酶/合成酶
固氮 Nitrogen fixation	glnA	谷氨酸-氨连接酶
	gltB	谷氨酸合酶
	narG	硝酸盐还原酶
	narH	硝酸盐还原酶
	narI	亚硝酸盐氧化还原酶
	nirA	铁氧还蛋白-亚硝酸盐还原酶
	cynT	碳酸酐酶
非核糖体肽 Non-ribosomal peptides	tycC	非核糖体肽合成酶
	dhbF	非核糖体肽合成酶
	mcyA	非核糖体肽合成酶
	ituB	非核糖体肽合成酶
	bacA	非核糖体肽合成酶

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