生物技术通报 ›› 2013, Vol. 0 ›› Issue (2): 15-21.

• 研究报告 • 上一篇    下一篇

斑马鱼在转基因动物研究中的应用

刘丽丽 王健 吴巍 王海胜 闫艳春   

  1. (中国农业科学院研究生院,北京 100081)
  • 收稿日期:2012-07-20 修回日期:2013-02-27 出版日期:2013-02-26 发布日期:2013-02-27
  • 作者简介:刘丽丽,女,硕士研究生,研究方向:微生物分子生物学与基因工程;E-mail :lilyinmay@163.com
  • 基金资助:
    中国高技术研究发展“863”计划(2008AA10Z402),国家自然科学基金项目(31170119),中国农业科学院基础研究基金项目(0042011006,0042012003)

Application of Zebrafish in Research of Transgenic Organisms

Liu Lili Wang Jian Wu Wei Wang Haisheng Yan Yanchun   

  1. (Graduate School of Chinese Academy of Agracultural Sciences,Beijing 100081)
  • Received:2012-07-20 Revised:2013-02-27 Published:2013-02-26 Online:2013-02-27

摘要: 近年来的研究表明,脊椎动物斑马鱼已发展成为一种十分重要的模式生物,在基因表达调控、发育与分化、形态发生、发病机理、药物筛选和环境检测等诸多研究领域都有重要的应用价值。在回顾模式生物应用与发展的基础上,着重概述斑马鱼作为模式生物的优越性、斑马鱼转基因技术的发展现状、转基因斑马鱼的应用领域,并提出斑马鱼转基因技术进一步发展可能面临的问题。

关键词: 模式生物, 斑马鱼, Danio rerio, 转基因动物, 应用

Abstract: The recent explosion of transgenic zebrafish lines in the literature demonstrates the value of this model system for detailed in vivo analysis of gene regulation, development and differentiation and morphogenetic movements, as well as drug screening and environment monitoring. After an outline of the application and development history of vital models from Escherichia coli to Musmusculus, the superiority of zebrafish as a model organism will be emphasized. Then this article will review the development history of transgenic technology in zebrafish and the uses of transgenic zebrafish. And finally problems in future development of transgenic zebrafish will be presented.

Key words: Model organism, Zebrafish, Danio rerio, Transgenic animal, Application