关键词: 新型氧肥, 土壤微生物总DNA, 16S, rDNA, DGGE

Abstract: We used Macropanax rosthornii soil as research subject, and water as the stress factor. The experiment was tested 36 days under the following three different conditions：A just was waterlogged；B was waterlogged with new oxygen fertilizer；C was normally watered without new oxygen fertilizer. Amplifying 16S rDNA genes of bacteria by PCR, subsequently the products of PCR were analyzed by DGGE on the sixth, twelfth, eighteenth, twenty-fourth, thirtieth and thirty-sixth day. The results indicate that at the same stage, with the prolongation of processing time, condition A and condition B’s DGGE bands similarity are reduced ；on the twelfth, eighteenth and twenty fourth day, the DGGE bands of condition B are more and brighter than condition A. The Shannon index of B is higher than A, and the difference presents the trend of “low-highlow”. The DGGE bands of B and C owns lower similarity during the same period. The diversity of different conditions presents differently during the detection time. Under the condition A, the similarity of soil bacteria diversity is generally lower at each detection time. Under the condition B, the similarity of soil bacteria diversity is higher and stable at each detection time. Under the condition C, the similarity of soil bacteria diversity is lower at each detection time. The research reveals the new oxygen fertilizer has a significant effect on waterlogged soil bacterial diversity.

Key words: New oxygen fertilizer, Soilmicrobial DNA, 16S, rDNA, DGGE