两株藏南地区土壤根瘤菌分类地位的确定

摘要/Abstract

摘要： 通过多相分类技术对分离自藏南地区土壤的两株根瘤菌进行了分类地位的确定。两株菌CNU8561007 与 CNU85000012 均为革兰氏阴性、好氧的杆状细菌。通过表型特征分析发现二者在多项生理指标上表现一致，由于两菌株来源于不同的地理位置，二者在表型特征上也存在少许差异。经过比较16S rDNA 序列发现，两供试菌株的序列相似性为100%，并且与 Rhizobum yanglingense，R.loessense，R.mongolense，R.gallicum 表现出了极高的相似性，4 种看家基因atpD，recA，glnII 和danK 的系统发育分析与16S rDNA 序列分析的结果相吻合，一致显示与两供试菌株遗传距离最近的模式种为Rhizobum yanglingense，通过 DNA 同源性分析和DNA G+C mol% 测定，最终将这两菌株归入到Rhizobum yanglingense 中，两菌株在BOX 指纹图谱上的差异表明了它们为同种内的不同菌株。

关键词: 根瘤菌, 16S, rDNA, 看家基因, DNA, 杂交, BOX-PCR

Abstract: Polyphasic taxonomy was adopted to determine the taxonomic status of two soil rhizobia from southern Tibet. Two Gramnegative, aerobic, rod-shaped bacteria, designated strains CNU8561007 and CNU85000012. The morphological studies demonstrated that the two test strains had similar features. The slight features differences between CNU8561007 and CNU85000012 might be related to the fact that they were isolated from different geographic regions. According to comparison of 16S rRNA gene sequences, strains CNU8561007 and CNU85000012 were identical and their closest phylogenetic relatives were Rhizobum yanglingense, R.loessense, R.mongolense and R.gallicum. Phylogenetic analysis based on four housekeeping genes（recA, atpD, glnII, and danK）which were in broad agreement with 16S rRNA gene sequence similarities indicated that the two isolates were closely related to Rhizobum yanglingense. The DNA G + C mol% and DNA -DNA hybridization provided supporting evidence that the two test strains belongs to the Rhizobum yanglingense and the BOX-PCR fingerprint profiles demonstrating that the two isolates were not clones of one strain.

Key words: Rhizobia 16S rDNA, Housekeeping genes, DNA-DNA hybridization, BOX-PCR

单辉辉李正韩素贞. 两株藏南地区土壤根瘤菌分类地位的确定[J]. 生物技术通报, 2013, 0(4): 158-166.

Shan Huihui, Li Zheng, Han Suzhen . The Determination of the Taxonomic Status of Two Soil Rhizobia from Southern Tibet[J]. Biotechnology Bulletin, 2013, 0(4): 158-166.

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