盐碱地星星草根cDNA文库的构建及初步分析

生物技术通报 ›› 2013, Vol. 0 ›› Issue (5): 69-72.

盐碱地星星草根cDNA文库的构建及初步分析

付畅, 王胜楠, 郭敏

(哈尔滨师范大学生命科学与技术学院，哈尔滨 150025)

收稿日期:2012-12-17 修回日期:2013-05-24 出版日期:2013-05-24 发布日期:2013-05-24
作者简介:付畅，博士，副教授，研究方向：生化与分子生物学；E-mail: fuchanghnu@yahoo.com.cn
基金资助:
哈尔滨师范大学青年学术骨干资助计划项目(09KXQ-01)

Construction and Preliminary Analysis of cDNA Library of Puccinellia tenuiflora Roots Grown in Saline-alkaline Land

Fu Chang, Wang Shengnan, Guo Min

(College of Life Sciences and Technology，Harbin Normal University，Harbin 150025)

Received:2012-12-17 Revised:2013-05-24 Published:2013-05-24 Online:2013-05-24

摘要/Abstract

摘要： 以盐碱地原位取材的星星草根为材料，利用SMART技术构建了cDNA文库。扩增后文库的滴度为1.747×10⁹ CFU/mL，插入片段分布在0.5-2 kb之间，重组率为92％。文库ESTs序列的初步分析表明，从盐碱地星星草根的cDNA文库中筛选到耐盐相关基因S-腺苷甲硫氨酸合成酶2基因和钙牵蛋白基因的EST片段。该文库可用于进一步从中筛选星星草耐盐基因。盐碱地星星草根cDNA文库的构建为揭示星星草耐盐分子机制、挖掘星星草耐盐基因、培养耐盐植物奠定了重要基础。

关键词: 盐碱地, 星星草, 根, cDNA文库, 表达序列标签

Abstract: Puccinellia tenuiflora roots gathered from saline-alkaline land were used to construct a cDNA library by using SMAT technology. The titer of the amplified cDNA library was 1.747×10⁹ CFU/mL, the inserted fragments were distributed from 0.5 kb to 2 kb, and the recombinant rate is 92%. Preliminary analysis of ESTs indicated that S-adenosylmethionine synthetase 2 gene and caltractin gene associated closely with plant salt-responsive reactions were screened in this cDNA library, which can be used to further screen salt-tolerant genes from Puccinellia tenuiflora. This cDNA library of Puccinellia tenuiflora roots laid important foundations for revealing salt tolerant mechanism in Puccinellia tenuiflora, mining salt tolerant genes, and cultivating engineered salt-tolerant plants.

Key words: Saline-alkaline land, Puccinellia tenuiflora, Roots, cDNA library, ESTs

付畅, 王胜楠, 郭敏. 盐碱地星星草根cDNA文库的构建及初步分析[J]. 生物技术通报, 2013, 0(5): 69-72.

Fu Chang, Wang Shengnan, Guo Min. Construction and Preliminary Analysis of cDNA Library of Puccinellia tenuiflora Roots Grown in Saline-alkaline Land[J]. Biotechnology Bulletin, 2013, 0(5): 69-72.

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(责任编辑李楠)