关键词: 小麦, 种子胚, 双向电泳

Abstract: For the comparison of different concentrations of wheat under salt stress seed germination stage embryo protein expression, “Jinmai -47” as the test material. Select the 3 concentrations of 0, 0.1 mol/L, 0.2 mol/L treated seeds, with the technology of 2-DE 3 concentrations under the embryo protein content changes have been studied. By PDQuest image analysis software analysis under salt stress protein changes, detected in normal seed embryo has 121 points, 0.1 mol/L under NaCl stress 32 protein spots abundance down, 21 protein spots increase in abundance；0.2 mol/L NaCl stress condition, is detected by a 46 point cut protein abundance, 16 protein spots abundance increase.Research results show that salt stress on seed germination of wheat embryos during part of the protein content and the expression have varying degrees of inhibition and activation.

Key words: Wheat, Seed embryo, Two-dimensional electrophoresis