反硝化聚磷菌的分离鉴定及其特性研究

生物技术通报 ›› 2013, Vol. 0 ›› Issue (5): 171-176.

反硝化聚磷菌的分离鉴定及其特性研究

张武刚¹, 洪武林², 郑春丽³

(1.上海清华紫光环境工程有限公司，上海 201204；2.上海立源水处理技术有限责任公司，上海 201108；3.内蒙古科技大学数理与生物工程学院，包头 014010)

收稿日期:2012-11-10 修回日期:2013-05-24 出版日期:2013-05-24 发布日期:2013-05-24
作者简介:张武刚，男，工程师，研究方向：水污染控制工程、应用微生物学；E-mail：wugang_zhang@gmail.com
基金资助:
国家自然科学基金项目(51264029)

Isolation，Identification and Characteristics of Denitrifying Polyphosphate-Accumulating Organisms

Zhang Wugang¹, Hong Wulin², Zheng Chunli³

(1. Shanghai Tsinghua Unisplendour Environmental Engineering Co.，Ltd，Shanghai 201204；2. Shanghai Liyuan Water Treatment Technology Co.，Ltd，Shanghai 201108；3. School of Mathematics，Physics and Biological Engineering，Inner Mongolia University of Science & Technology，Baotou 014010)

Received:2012-11-10 Revised:2013-05-24 Published:2013-05-24 Online:2013-05-24

摘要/Abstract

摘要： 通过除磷试验、反硝化产气试验以及异染颗粒和聚-β-羟基丁酸(PHB)颗粒染色试验，从内蒙古乌梁素海湖水中筛选出一株高效反硝化聚磷菌株P1-1。经过形态、生理生化试验、BIOLOG碳源利用分析以及16S rRNA基因序列分析，鉴定该菌株为荧光假单胞菌(Pseudomonas fluorescens)。测定了菌株P1-1的生长曲线，主要研究了温度、pH对菌株P1-1的生长和除磷率的影响，结果表明培养12 h以后菌株P1-1就开始进入稳定期；菌株P1-1的最佳生长和除磷条件为温度30℃，pH8.0。

关键词: 反硝化聚磷菌, 筛选, 鉴定, 温度, pH

Abstract: Being screened by a phosphate uptake experiment, where nitrates were denitrified to gas and through inspection of metachromatic and Polyphydroxybutyrate (PHB) granules, denitrifying phosphate-accumulating organisms (DNPAOs) P1-1 was isolated from a water sample in Lake Wuliangsuhai of Inner Mongolia. According to its morphological, physiological characteristics, BIOLOG identification and the analysis of its 16S rRNA sequence, it was identified as a strain of Pseudomonas fluorescens. The growth curve shows that strain P1-1 enters stationary phase in 12 hours’ training. Meanwhile, some important factors, temperature and pH value were studied to evaluate their effect on the growth and phosphorus removal ratios of strain P1-1. The results showed that the optimal conditions for the strain’s growth and phosphorus removal are 30℃ and pH8.0.

Key words: Denitrifying phosphate-accumulating organisms (DNPAOs) , Screening Identification, Temperature pH

张武刚, 洪武林, 郑春丽. 反硝化聚磷菌的分离鉴定及其特性研究[J]. 生物技术通报, 2013, 0(5): 171-176.

Zhang Wugang, Hong Wulin, Zheng Chunli. Isolation，Identification and Characteristics of Denitrifying Polyphosphate-Accumulating Organisms[J]. Biotechnology Bulletin, 2013, 0(5): 171-176.

参考文献

[1] 吕志堂, 纪翠平, 苏强, 等. 3株反硝化聚磷菌的分离与鉴定[J].环境工程学报, 2009, 3(8)：1405-1048.
[2] Lee DS, Jeon CO, Park JM. Biological nitrogen removal with enhanced phosphate uptake in a sequencing batch reactor using single sludge system [J]. Water Research, 2001, 35(16)：3968-3976.
[3] Ahn J, Daidou T, Tsuneda S, et al. Characterization of denitrifying phosphate-accumulating organisms cultivated under different electron acceptor conditions using polymerase chain reaction-denaturing gradient gel electrophoresis assay [J]. Water Research, 2002, 36(2)：403-412.
[4] Kuba T, Van Loosdrecht MCM, Heijnen JJ. Phosphorus and nitrogen removal with minimal COD requirement by integration of denitrify dephosphatation and nitrification in a two-sludge system [J]. Water Research, 1996, 30(7)：1702-1710.
[5] Merzouki M, Bernet N, Delgenes JP, et al. Bioloical denitrifying phosphorus removal in SBR：effect of added nitrate concentration and sludge retention time [J]. Water Science and Technology, 2001, 43(3)：191-194.
[6] 马放, 王春丽, 王立立.高效反硝化聚磷菌株的筛选及其生物学特性[J].哈尔滨工程大学学报, 2007, 28(6)：631-635.
[7] 杨志愿, 邱业先, 李孝坤, 等.几株反硝化聚磷菌的筛选及其生理生化特性的鉴定[J].苏州科技学院学报, 2009, 26(3)：42-48.
[8] Merzouki M, Delgenes JP, Bernet N, et al. Polyphosphate-accumulating and denitrifying bacteria isolated from anaerobic-anoxic and anaerobic-aerobic sequencing batch reactors [J]. Current Microbiology, 1999, 3(8)：9-17.
[9] 罗宁, 罗固源, 吉方英, 等.新型双泥生物反硝化除磷脱氮系统中微生物的组成[J].给水排水, 2003, 29(8)：33-35.
[10] Buchanan RE, Gibbons NE.伯杰细菌鉴定手册(第8版)[M].北京：科学出版社, 1984：274-313.
[11] Liu JS, Xie XH, Xiao SM. Isolation of Leptospirillum ferriphilum by single-layered solid medium [J]. Journal of Central South University of Technology, 2007, 14(4)：467-473.
[12] Thompson JD, Gibson TJ, Plewniak F, et al. The CLUSTAL X windows interface：flexible strategies for multiple sequence alignment aided by quality analysis tools [J]. Nucleic Acids Research, 1997, 25(24)：4876-4882.
[13] Kumar S, Tamura K, Nei M. MEGA3：integrated software for molecular evolutionary genetics analysis and sequence alignment [J]. Briefings in Bioinformatics, 2004, 5(2)：150-163.
[14] Vlekke G, Comeau Y, Oldham WK. Biological phosphate removal from wastewater with oxygen or nitrate in sequencing batch reactors [J]. Environmental Technology Letters, 1988, 9(8)：791-796.
(责任编辑马鑫)

[1]	周璐祺, 崔婷茹, 郝楠, 赵雨薇, 赵斌, 刘颖超. 化学蛋白质组学在天然产物分子靶标鉴定中的应用[J]. 生物技术通报, 2023, 39(9): 12-26.
[2]	温晓蕾, 李建嫄, 李娜, 张娜, 杨文香. 小麦叶锈菌与小麦互作的酵母双杂交cDNA文库构建与应用[J]. 生物技术通报, 2023, 39(9): 136-146.
[3]	吴巧茵, 施友志, 李林林, 彭政, 谭再钰, 刘利平, 张娟, 潘勇. 类胡萝卜素降解菌株的原位筛选及其在雪茄提质增香中的应用[J]. 生物技术通报, 2023, 39(9): 192-201.
[4]	江海溶, 崔若琪, 王玥, 白淼, 张明露, 任连海. NH₃和H₂S降解功能菌的分离鉴定及降解特性研究[J]. 生物技术通报, 2023, 39(9): 246-254.
[5]	薛宁, 王瑾, 李世新, 刘叶, 程海娇, 张玥, 毛雨丰, 王猛. 多基因同步调控结合高通量筛选构建高产L-苯丙氨酸的谷氨酸棒杆菌工程菌株[J]. 生物技术通报, 2023, 39(9): 268-280.
[6]	饶紫环, 谢志雄. 一株Olivibacter jilunii 纤维素降解菌株的分离鉴定与降解能力分析[J]. 生物技术通报, 2023, 39(8): 283-290.
[7]	马俊秀, 吴皓琼, 姜威, 闫更轩, 胡基华, 张淑梅. 蔬菜软腐病菌广谱拮抗细菌菌株筛选鉴定及防效研究[J]. 生物技术通报, 2023, 39(7): 228-240.
[8]	谢东, 汪流伟, 李宁健, 李泽霖, 徐子航, 张庆华. 一株多功能菌株的发掘、鉴定及解磷条件优化[J]. 生物技术通报, 2023, 39(7): 241-253.
[9]	袁野, 周佳, 屈建航, 张博源, 罗宇, 李海峰. 高效反硝化聚磷菌的筛选及其脱氮除磷条件和性能研究[J]. 生物技术通报, 2023, 39(7): 266-276.
[10]	游子娟, 陈汉林, 邓辅财. 鱼皮生物活性肽的提取及功能活性研究进展[J]. 生物技术通报, 2023, 39(7): 91-104.
[11]	李典典, 粟元, 李洁, 许文涛, 朱龙佼. 抗菌适配体的筛选与应用进展[J]. 生物技术通报, 2023, 39(6): 126-132.
[12]	张路阳, 韩文龙, 徐晓雯, 姚健, 李芳芳, 田效园, 张智强. 烟草TCP基因家族的鉴定及表达分析[J]. 生物技术通报, 2023, 39(6): 248-258.
[13]	董聪, 高庆华, 王玥, 罗同阳, 王庆庆. 基于联合策略提高FAD依赖的葡萄糖脱氢酶的酵母表达[J]. 生物技术通报, 2023, 39(6): 316-324.
[14]	王一帆, 候林慧, 常永春, 杨亚杰, 陈天, 赵祝跃, 荣二花, 吴玉香. 陆地棉与拟似棉异源六倍体的合成与性状鉴定[J]. 生物技术通报, 2023, 39(5): 168-176.
[15]	车永梅, 郭艳苹, 刘广超, 叶青, 李雅华, 赵方贵, 刘新. 菌株C8和B4的分离鉴定及其耐盐促生效果和机制[J]. 生物技术通报, 2023, 39(5): 276-285.