青贮饲料中优良乳酸菌的分离鉴定及其生物学特性研究

生物技术通报 ›› 2013, Vol. 0 ›› Issue (5): 177-183.

青贮饲料中优良乳酸菌的分离鉴定及其生物学特性研究

何轶群¹, 雷赵民², 吴润¹, 万学瑞¹, 刁小龙¹, 艾文娜¹

(1.甘肃农业大学动物医学院，兰州 730070；2.甘肃农业大学动物科学技术学院，兰州 730070)

收稿日期:2012-10-29 修回日期:2013-05-24 出版日期:2013-05-24 发布日期:2013-05-24
作者简介:何轶群，男，硕士研究生，研究方向：兽医微生物与免疫学；E-mail：heyiqunsky@126.com
基金资助:
甘肃省农业生物技术研究与应用开发项目(GNSW-2010-05)，甘肃省农业生物技术研究与应用开发项目(GNSW-2012-25)，兰州市攻关及产业化项目(2012-2-159)，甘肃省科技支撑计划(1204NKCA103)

Isolation and Identification of Excellent Lactic Acid Bacteria from Silage and Its Biological Characteristics Research

He Yiqun¹, Lei Zhaomin², Wu Run¹, Wan Xuerui¹, Diao Xiaolong¹, Ai Wenna¹

(1. College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070；2. College of Animal Science and Technology，Gansu Agricultural University，Lanzhou 730070)

Received:2012-10-29 Revised:2013-05-24 Published:2013-05-24 Online:2013-05-24
About author:吴润，男，教授，博士生导师，研究方向：兽医微生物与免疫学；E-mail：wurun@gsau.edu.cn

摘要/Abstract

摘要： 为筛选优良的青贮饲料添加剂，采用平板分离法从青贮饲料中分离乳酸菌，通过细菌形态学、生理生化特征鉴定和16S rRNA基因序列分析相结合的方法对分离出的细菌进行鉴定。通过产酸性试验和抑菌试验对分离出来的12株乳酸菌进行筛选，并对筛选出的6株乳酸菌进行生物学特性比较。结果显示，5株为戊糖片球菌，4株为植物乳杆菌，1株为发酵乳杆菌，1株为肠系膜明串珠菌肠膜亚种，1株为屎肠球菌；菌株B1-6、B1-7、B2-3、B2-8、B3-1、B5-2的产酸能力和抑菌效果较好；菌株B1-7和B5-2生长最快，8 h后进入稳定期，其余菌株14 h后进入稳定期；菌株B1-7在培养温度高于45℃，培养基pH>10和含盐量>0.08 g/mL时不再生长，其余菌株均具有良好的抗逆性具备青贮潜力，可作为秸秆青贮的生物添加剂做进一步研究。

关键词: 乳酸菌, 16S, rRNA, 筛选, 生物学特性

Abstract: To screen excellent microbials for silage additive usage, 12 bacteria strains were isolated from the silage. According to the results of morphological observation, physiological and biochemical tests, and sequence analysis of the 16S rRNA gene of the strains isolated, 6 strains were screened and compared through acid yield tests and bacteriostatic tests. The results showed 5 strains were Pediococcus pentosaceus, 4 strains were Lactobacillus plantarum, 1 strain was Lactobacillus fermentum, 1 strain was Leuconostoc mesenteroides subsp. mesenteroides, 1 strain was Enterococcus faecium；Strain B1-7 and B5-2 which reached a stable period after 8 h were fastest-growing, others were 14 h；The B1-7 stain no longer grow at temperature higher than 45℃, medium pH>10, salinity>0.08 g/mL. Other stains could be used as bio-straw silage additives to do further research which showed strong resistance and silage potential.

Key words: Lactic acid bacteria 16S rRNA, Screen, Biological characteristics

何轶群, 雷赵民, 吴润, 万学瑞, 刁小龙, 艾文娜. 青贮饲料中优良乳酸菌的分离鉴定及其生物学特性研究[J]. 生物技术通报, 2013, 0(5): 177-183.

He Yiqun, Lei Zhaomin, Wu Run, Wan Xuerui, Diao Xiaolong, Ai Wenna. Isolation and Identification of Excellent Lactic Acid Bacteria from Silage and Its Biological Characteristics Research[J]. Biotechnology Bulletin, 2013, 0(5): 177-183.

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