免疫胶体金试纸条快速检测贝类体内的腹泻性贝毒

生物技术通报 ›› 2013, Vol. 0 ›› Issue (6): 221-225.

免疫胶体金试纸条快速检测贝类体内的腹泻性贝毒

张洋, 贾睿, 何培民

(上海海洋大学水产与生命学院, 上海 201306)

收稿日期:2013-06-20 修回日期:2013-06-20 出版日期:2013-06-20 发布日期:2013-06-20
作者简介:张洋, 女, 硕士研究生, 研究方向: 海洋藻毒素快速免疫检测;E-mail: zymiya@gmail.com
基金资助:
上海市科委项目(10391901900), 国家科技支撑计划课题(2012BAC07B03), 上海市教委科研创新项目(11YZ149), 教育部留学回国人员科研启动基金, 国家海洋局东海分局开放基金(MATHAB20100205, MATHAB 20100309), 上海市教委海洋生物重点学科(J50701)

Rapid Determination of Diarrhetic Shellfish Poisoning Toxins in Shellfish Samples by Immune Colloidal Gold Strip

Zhang Yang, Jia Rui, He Peimin

(College of life Science and Shanghai Ocean University, Shanghai 201306)

Received:2013-06-20 Revised:2013-06-20 Published:2013-06-20 Online:2013-06-20

摘要/Abstract

摘要： 首次应用自行研制开发的免疫胶体金试纸条, 对上海市海产品市场上的双壳贝类腹泻性贝毒的主要成分大田软海绵酸(Okadaic Acid, OA) 的污染情况进行了调查。自2011年5月至2011年10月, 共采集69份贝类样品, 经处理后, 通过OA免疫胶体金试纸条检测、酶联免疫吸附测定(ELISA) 验证。结果显示, 免疫胶体金试纸条共检测出11份样品含有OA, 检出率为15.9％。这11份样品为5份贻贝(Mytilus) , 4份海湾扇贝(Argopecten irradias) , 1份近江牡蛎(Crassostrea rivularis) , 1份黄泥螺(Bullacta exarata) 。经ELISA检测方法验证表明, 免疫胶体金试纸条的11份阳性样品中, OA含量均在15 μg/100 g贝肉以上, 结果与ELISA检测结果100%吻合。应用免疫胶体金试纸条检测贝类样品, 从样品处理到观测结果, 时间在30 min之内, 且检测结果准确可靠, 没有假阳性的出现, 基本满足对OA安全食用标准的检测要求, 可用于现场快速检测, 在海洋环境监测及海产品检验检疫方面具有较好的应用价值。

关键词: 腹泻性贝毒, 大田软海绵酸, 免疫胶体金试纸条, 快速检测

Abstract: In the present study we determined the Okadaic Acid(OA), the main pathotoxin of diarrhetic shellfish poisoning toxins(DSPs), in shellfish samples in Shanghai sea food markets by the immune colloidal gold strip for the first time which is researched and developed by our lab. There are 69 samples which were collected from May 2011 to October 2011 for each month from the seafood market. The samples were tested by OA immune colloidal gold strip and enzyme-linked immunosorbnent assay(ELISA)after a series of processing. The result showed that there are 11 positive samples contain OA for OA Immune colloidal gold strip, and the positive rate is 15.9％. The 11 positive shellfish samples were Mytilus edulis(n=5), Argopecten irradians(n=4), Ostrea(n=1)and Bullacta exarata(Philippi)(n=1). The result of ELISA showed that the 11 samples which were tested positive by immune colloidal gold strip contain the OA, and the concentration of OA was quantified higher than 15 μg/100 g. The correlation between OA concentration determined via Immune colloidal gold strip and ELISA is 100%. The time of determining the shellfish samples using the OA immune colloidal gold strip is within 30 min from collecting the samples to get the result, and the result is accurate with no false positives. In the present study, immune colloidal gold strips test were shown to be an effective tool for rapid screening in marine environmental monitoring and seafood quarantine and inspection with the limit of detection meeting the safe threshold of OA in shellfish.

Key words: Diarrhetic shellfish poisoning, Okadaic acid, Immune colloidal gold strip, Rapid determination

张洋, 贾睿, 何培民. 免疫胶体金试纸条快速检测贝类体内的腹泻性贝毒[J]. 生物技术通报, 2013, 0(6): 221-225.

Zhang Yang, Jia Rui, He Peimin . Rapid Determination of Diarrhetic Shellfish Poisoning Toxins in Shellfish Samples by Immune Colloidal Gold Strip[J]. Biotechnology Bulletin, 2013, 0(6): 221-225.

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