Effects of Nitrogen Fertilizer on the Expression of nifH Gene of Endophytic Azotobacter in Sugarcane Leaves

doi:10.13560/j.cnki.biotech.bull.1985.20170002

Abstract

Abstract: In order to investigate the effects of nitrogen fertilizer on the expression of nifH gene of endophytic diazotrophs in sugarcane leaves，we carried out a field experiment with and without nitrogen fertilization，and used RT-PCR and Western blot technology to analyze the differences of nifH expression and the relative expression quantity of nitrogenase in 3 sugarcane varieties，GXB9，ROC22 and GT11. The results showed that the expressions of nifH gene in endogenous azotobacter were detected at gene and protein levels in the leaves of sugarcane growing in field both with nitrogen fertilization and without nitrogen fertilization. Nitrogen fertilization treatment inhibited or decreased the expression of nifH gene in nitrogen-fixing bacteria，but it was beneficial to the expression of nifH at the protein level. The relative expressions of the nifH gene in the leaves of three sugarcane cultivars were all higher under nitrogen application than the control without nitrogen application；especially，the difference was significant for cultivar ROC22.

Key words: sugarcane, endogenous azotobacter, nitrogenase, nifH

LI Jia-hui, YUAN Dan, LU Jian-ming, YANG Li-tao, LI Yang-rui ,XING Yong-xiu. Effects of Nitrogen Fertilizer on the Expression of nifH Gene of Endophytic Azotobacter in Sugarcane Leaves[J]. Biotechnology Bulletin, 2017, 33(7): 100-106.

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