Characteristics of Endophytic Fungal and Bacterial Community in the Stalks of Sugarcane Cultivars Resistant to Ratoon Stunting Disease

doi:10.13560/j.cnki.biotech.bull.1985.2021-1197

Abstract

Abstract:

By analyzing the structural characteristics of endophytic fungi and bacteria in the stalks of sugarcane varieties resistant to ratoon stunting disease（RSD）,the aim is to understand the roles of endophytic fungi and bacteria in the resistance of sugarcane,so as to provide theoretical basis for ecological control of RSD. Using Illumina MiSeq high-throughput sequencing method,the communities,diversity index,and differences of endophytic bacteria and fungi between susceptible variety YT60 and resistant variety HoCP95988 were compared. The Alpha diversity indexes of endophytic fungi and bacteria in the stalks of susceptible cultivar YT60 was higher than that of resistant cultivar HoCP95988,indicating more species composition. The endophytic bacteria mainly distributed in Proteobacteria,while Ascomycota and Basidiomycota were the dominant phyla of endophytic fungi. The species compositions of 34 genera in 4 phyla Planctomycetes,Chloroflexi,Gemmatimonadetes,and Tenericutes between susceptible and resistant varieties were significantly different. Among them,the relative abundance of different species was higher in susceptible variety YT60. The diversity of endophytic fungal and bacterial communities in the stalks of sugarcane varieties resistant to RSD were relatively rich,and there were significant differences in the composition. Meanwhile,the classification information of different species provided clues to explore the biocontrol microbial resources for control RSD.

Key words: sugarcane, ratoon stunting disease, endophytic fungi, endophytic bacteria, diversity index, population composition

GAO Xiao-ning, LIU Rui, WU Zi-lin, WU Jia-yun. Characteristics of Endophytic Fungal and Bacterial Community in the Stalks of Sugarcane Cultivars Resistant to Ratoon Stunting Disease[J]. Biotechnology Bulletin, 2022, 38(6): 166-173.

Figures/Tables 5

Table 1 Sequencing data, ASVs and diversity index of endophytic fungi and bacteria in the stalks of different sugarcane cultivars

	样品名称 Sample name	高质量序列量 Number of high quality sequence	ASVs	Chao1	Observed species	Shannon	Simpson
内生细菌 Endophytic bacteria	YT60	552269	3061	780.91±393.51	777.88±392.31	4.94±1.96	0.82±0.16
内生细菌 Endophytic bacteria	HoCP95988	569775	1365	360.55±105.85	358.20±104.80	4.41±0.57	0.88±0.05
内生真菌Endophytic fungi	YT60	520732	176	45.59±20.76	45.30±20.63	4.09±1.07	0.87±0.12
内生真菌Endophytic fungi	HoCP95988	480771	96	23.30±15.26	22.04±12.52	2.62±0.70	0.73±0.15

Fig. 2 Dominant genera detected from endophytic bacterial（A）and fungal（B）community in the stalks of different sugarcane cultivars

Fig. 1 Dominant phyla detected from endophytic bacterial（A）and fungal（B）community in the stalks of different suga-rcane cultivars

Fig. 3 ASV Venn diagram of endophytic bacteria（A）and fungi（B）in the stalks of different sugarcane cultivars

Fig. 4 LEfSe cladogram of endophytic bacteria in the stalks of different sugarcane cultivars Blue：YT60;Red：HoCP95988

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