The Expression Analysis of Sucrose Transporter Genes in Disease Free Sugarcane

doi:10.13560/j.cnki.biotech.bull.1985.2018-0626

Abstract

Abstract: Sucrose transporters are closely correlative with sugar storage and crop yield formation.It is helpful to clarify the molecular mechanism on increasing yield and sucrose content by analyzing the expression pattern of sucrose transporter genes in disease free sugarcane seedlings. The expression differences of three sucrose transporter genes,SUT1,SUT4 and SUT6 were analyzed between sugarcane diseases free seedlings and ordinary seedlings during the whole growth period by Real time PCR. The results showed that SUT1,SUT4 and SUT6 were up regulated in immature leaves of diseases free sugarcane plants at seedling growth stage,however SUT1 and SUT4 were down regulated and SUT6 were up regulated in mature leaves of diseases free sugarcane plants at seedling growth stage. SUT1,SUT4 and SUT6 were up regulated in leaves of diseases free sugarcane plants at tillering growth stage. SUT4 and SUT6 were up regulated in immature leaves and internodes of diseases free sugarcane plants at elongation growth stage. The expression of SUT1 was not different between diseases free and ordinary sugarcane plants at elongation growth stage. Moreover,SUT1,SUT4 and SUT6 were up regulated in leaves and immature internodes of diseases free sugarcane plants at mature stage. In conclusion,SUT1,SUT4 and SUT6 were up regulated in actively metabolism and rapid growth tissues of diseases free sugarcane plants. It postulated that the up-regulated sucrose transporter genes by disease-free treatment promoted sucrose partition and utilization to ultimately improve the cane yield and sucrose content.

Key words: sugarcane, sucrose transporter, gene expression, disease free seedlings

ZHAO Ting-ting, WANG Jun-gang, YANG Ben-peng, SHEN Lin-bo, FENG Xiao-yan, WANG Wen-zhi, FENG Cui-lian, XIONG Guo-ru, ZHANG Shu-zhen. The Expression Analysis of Sucrose Transporter Genes in Disease Free Sugarcane[J]. Biotechnology Bulletin, 2018, 34(12): 125-131.

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