Cloning and Bioinformatics Analysis of the CDS of Yak AQP4 Gene

doi:10.13560/j.cnki.biotech.bull.1985.2015.02.019

Abstract

Abstract: A coding region sequence of yak AQP4 was cloned by molecular cloning technique. Some characters of the AQP4 gene and encoded protein sequences were predicted and analyzed by the methods of bioinformatics in the following aspects as the general physical and chemical properties, hydrophobicity, transmembrane structure and secondary structure. Results showed that the full-length of yak AQP4 contains a complete ORF（966 bp） encoding 322 amino acid. The putative molecular weight and theory isoelectric point of AQP4 gene in yak were 34.69 kD and 7.59, respectively. The protein encoded by yak AQP4 contains six transmembrane α-helices, and it is a hydrophobic protein. The second structures are mainly composed of α-helix, extended strand and random coil. Deduced amino acid sequences of AQP4 gene between yak and Bos Taurus, Ovis aries, Susscrofa and other species were high on homology and the phylogenetic distance consistent with their genetic relationship.

Key words: yak, Aquaporin 4（AQP4）, gene, CDS region, molecular cloning, bioinformatics

Liu Jianfeng, Ding Yanping, Hou Boru, Wang Jianlin, Shao Baoping. Cloning and Bioinformatics Analysis of the CDS of Yak AQP4 Gene[J]. Biotechnology Bulletin, 2015, 31(2): 129-134.

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