Progress in Methodology of Bacterial Noncoding RNA Research Based on High-throughput Sequencing

doi:10.13560/j.cnki.biotech.bull.1985.2015.03.015

Abstract

Abstract: Bacterial noncoding RNA refers to the nucleic acid molecules that do not encode proteins, but play roles in the regulation in the form of RNA in bacteria. Application of high-throughput sequencing technology has greatly advanced the discovery of non-coding RNA in a variety of bacterial species. However, due to the lack of enough knowledge on the characteristics of non-coding RNA in bacteria, the bioinformatics analysis of the sequencing data are still defective. This article describes two main techniquesthat applied high-throughput sequencing to be used in the researches on bacterial non-coding RNA：RNA-seq and dRNA-seq. The existing bioinformatics methods for screening of non-coding RNA are reviewed, and the recommendations for improving the bioinformatics strategy in this field are proposed.

Key words: bacteria, non-coding RNA, high-throughput sequencing, bioinformatics

Guo Mingzhang, Xu Wentao. Progress in Methodology of Bacterial Noncoding RNA Research Based on High-throughput Sequencing[J]. Biotechnology Bulletin, 2015, 31(4): 99-104.

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