Genetic Analysis and Gene Mapping of a Dominant Rolled Leaf Mutant z2 in Rice

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.014

Abstract

Abstract: Moderate rolled leaf is significant in ideotype breeding of rice. An outward rolled leaf mutant z2 was discovered from the rice T-DNA insertion mutant library, and its leaves became curly during tillering stage. Compared with the wild type, there was no significant difference in its main agronomic traits, and its photosynthetic was more efficient. Paraffin sections showed that the number of bulliform cells(8-9)in the mutant was higher than that in the wild type(4-5). Through successive selfing, phenotype of z2 was stable and was controlled by a pair of dominant nuclear genes. We made the combination of z2/Dular to establish F2 mapping population, and used map-based cloning method to locate the gene on rice chromosome 2 between maker InDel1812 and InDel1870 with 580 kb physical distance.

Key words: rice(Oryza sativa L.), rolled leaf mutant, genetic analysis, map-based cloning

Zhang Longdi, Wang Yanwei, Zhang Zhiguo, Wu Jinxia. Genetic Analysis and Gene Mapping of a Dominant Rolled Leaf Mutant z2 in Rice[J]. Biotechnology Bulletin, 2015, 31(6): 100-105.

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