Construction of Root Expression Vector of Brassinosteroid Gene BAS1 and Genetic Transformation into Tobacco

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.015

Abstract

Abstract: In order to allow brassinosteroid gene BAS1 overexpress in root, the root expression vector pCAMBIA2301-RB7-rbc with root promoter TobRB7 was constructed. Then inactivated gene BAS1 was integrated into the root overexpression vector pCAMBIA2301-RB7-BAS1-rbc, and they were transformed into tobacco by Agrobactrium tumefaciems infection. With the verification by PCR and RT-PCR, the target gene BAS1 was transformed into tobacco genome successfully, and expressed in root；the expression of brassinosteroid receptor kinase gene BRI1 in roots of transgenic plants was affected.

Key words: BAS1 gene, construction of root expression vector, transgenic plants, expression

Shu Hongmei, Guo Shuqiao, Gong Yuanyong, Ni Wanchao. Construction of Root Expression Vector of Brassinosteroid Gene BAS1 and Genetic Transformation into Tobacco[J]. Biotechnology Bulletin, 2015, 31(6): 106-110.

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