Cloning and Expression Analysis of a Transferrin Receptor 1 Gene from Swamp Eel, Monopterus albus

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.019

Abstract

Abstract: For investigating the function and tissue expression specificity of transferrin receptor 1 gene(TfR1)from swamp eel, TfR1 gene was isolated from the liver of swamp eel using homologous cloning and RACE techniques, the bioinformatics method was used to analyze the putative amino acid sequence, and semi-quantitative RT-PCR was used to study the expression of TfR1 gene in different tissues. The results showed that the full length of cDNA(GenBank accession number：KF819396)was 2 839 bp including a 134 bp 5' un-translated region(UTR)and a 380 bp 3' UTR, and it encoded a polypeptide of 774 amino acids. Sequence alignment by amino acid indicated that the inferred amino acid sequence of TfR1 from swamp eel shared high identity with those of other teleosts in 55.68%-68.41%. Semi-quantitative RT-PCR analyses demonstrated that the TfR1 transcripts were significantly varied in different tissues, the highest in blood cells, moderate in kidney, spleen and intestine, and little in liver, stomach, skin, brain, heart and muscle.

Key words: swamp eel, transferrin receptor 1(TfR1), RACE, RT-PCR

Jiang Ao, Li Wei. Cloning and Expression Analysis of a Transferrin Receptor 1 Gene from Swamp Eel, Monopterus albus[J]. Biotechnology Bulletin, 2015, 31(7): 132-137.

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