Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (2): 129-137.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0471

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Correlation Detween the Dynamic Changes of Morphological Structure and Molecular Weight of Coprinus comatus Extracellular Polysaccharides and Their Antioxidant Activity

WANG Shan-shan(), SUN Min, WANG Yong-xia, LI Wei-dong, HAN Chun-chao()   

  1. School of Pharmacy,Shandong University of Traditional Chinese Medicine,Ji’nan 250355
  • Received:2020-04-22 Online:2021-02-26 Published:2021-02-26
  • Contact: HAN Chun-chao E-mail:17862986901@163.com;chunchaoh@126.com

Abstract:

Detecting the structure of Coprinus comatus polysaccharides and exploring the relationship between structure and activity are of great significance for exploring its efficacy in depth. Three Coprinus comatus extracellular crude polysaccharides with fermentation times of 72 h,96 h and 120 h were prepared,and their monosaccharide composition were determined by the method of PMP pre-column derivatization combined with HPLC. The results showed that the extracellular polysaccharides fermented for 72 h,96 h and 120 h were all composed of D-mannose,L-rhamnose,D-glucose,D-galactose,and D-arabinose,but the relative molar ratio of each monosaccharide was different. Scanning electron microscopy and atomic force microscopy were used to analyze the morphology structure of polysaccharides,and the results showed that the plate-like structure of polysaccharides fermented 120 h was more regular and the molecules aggregation was stronger. High performance gel filtration chromatography was employed to analyze the molecular weight distribution,and the results demonstrated that the molecular weight of polysaccharides fermented for 120 h was larger and the distribution range was wider. In vitro antioxidant test results suggested that the polysaccharide fermented for 120 h displayed stronger antioxidant activity than that of polysaccharides fermented for 72 h and 96 h at the same concentration. The results indicate that the antioxidant activity of C. comatus extracellular polysaccharide may be related to its morphological structure and molecular weight distribution.

Key words: Coprinus comatus extracellular polysaccharides, monosaccharide analysis, morphological structure, molecular weight, antioxidant