Correlations Between Sucrose Accumulation and Activities of SPS, SS at Processing Maturing Stage of Sugarcane

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.014

Abstract

Abstract: The correlations between sucrose accumulation and activities of 3 enzymes viz, sucrose phosphate synthase(SPS), sucrose synthase in the synthesis direction(SS-s), and sucrose synthase in the cleavage direction(SS-c)were studied using the leaf+1 and 7 internodes of early-maturing cultivar GT28 and early-intermediate-maturing cultivar ROC22 at processing maturing stage of sugarcane. The results indicated that the activities of SPS, SS-s and SS-c in the leaf+1 were higher than those in stalks of both GT28 and ROC22, suggesting that the leaf+1 was vigorous and active for physiological metabolism, which was the substantial basis for continuous sucrose accumulation in stalks. The SPS activity in internode was significantly and positively correlated with the brix, total soluble sugar and sucrose content of internode, and it was higher in GT28 than in ROC22. Meanwhile, SS-s activity was significantly and negatively correlated with the brix and sucrose content in internode, and it was lower in GT28 than in ROC22. However, SS-c was significantly and negatively correlated with brix, total soluble sugar and sucrose content in the internode of ROC22, while positively in GT28 although it was not significant statistically. This suggests that SPS and SS-s activities promote the sucrose accumulation in the immature internodes；with the internode maturing, sucrose content might be an important regulatory factor to convert the SS activity from synthesis to cleavage direction.

Key words: sugarcane, sucrose phosphate synthase, sucrose synthase, sucrose accumulation, correlation analysis

Niu Junqi, Huang Jingli, Zhao Wenhui, Yang Litao, Li Yangrui. Correlations Between Sucrose Accumulation and Activities of SPS, SS at Processing Maturing Stage of Sugarcane[J]. Biotechnology Bulletin, 2015, 31(9): 105-110.

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