Comparison of the Superantigen Sensitivity on Staphylococcal Enterotoxins C2 in Guinea Pigs and BALB/c Mice

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.032

Abstract

Abstract: This work is to analyze and compare the superantigen sensitivity on staphylococcal enterotoxins C2(SEC2)in guinea pigs and BALB/c mice, and determine the model animal for the researches of superantigen sensitivity on SEC2. In vitro, peripheral blood mononuclear cell(PBMC)and splenocytes from guinea pigs and BALB/c mice were respectively stimulated with SEC2 at gradient concentration, and the cell proliferation was tested by MTS assay；In vivo, the changes of temperature and weight of guinea pigs and mice were measured at interval of 3 d after the intraperitoneal injection of SEC2. Meanwhile, the serums were collected at interval of 7 d to detect the levels of IL-4, IFN-γ, IL-2 and TNF-α by ELISA. SEC2 of different concentrations significantly stimulated the proliferation of PBMC and splenocytes from BALB/c mouse(P < 0.05), but not so from guinea pigs(P > 0.05). After intraperitoneal injection of SEC2, there was no significant difference in body temperature of both guinea pigs and mice(P > 0.05)compared to the control. In the later phase of the experiment, the body weights of guinea pigs in SEC2 group were significantly lower than those in control group(P < 0.05). And the body weights of mice in SEC2 group were also significantly lower than those in control group during day 3 to 30, but recovered to normal level at the end of the experiment(P > 0.05). The cytokines IL-4, IFN-γ, IL-2 and TNF-α in serum of mice of SEC2 group were significantly elevated compared with in control group(P < 0.05). However, there was no significant difference in guinea pigs administrated with SEC2 and physiological saline(P > 0.05). In conclusion, comparing with guinea pigs, BALB/c mice are more sensitive to the stimulation of SEC2 with promising dose-dependent and time-dependent manner. Therefore, BALB/c mouse is a suitable model animal for the researches of superantigen activity.

Key words: guinea pig, mouse, staphylococcal enterotoxins C2

Meng Beiqian, Zhang Huiwen, Zhang Guojun, Xu Mingkai, Li Xu, Zhang Chenggong. Comparison of the Superantigen Sensitivity on Staphylococcal Enterotoxins C2 in Guinea Pigs and BALB/c Mice[J]. Biotechnology Bulletin, 2015, 31(9): 224-231.

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