Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (9): 224-231.doi: 10.13560/j.cnki.biotech.bull.1985.2015.09.032

• Research report • Previous Articles     Next Articles

Comparison of the Superantigen Sensitivity on Staphylococcal Enterotoxins C2 in Guinea Pigs and BALB/c Mice

Meng Beiqian1,2, Zhang Huiwen2, Zhang Guojun1,2, Xu Mingkai2, Li Xu2, Zhang Chenggong1   

  1. (1. University of Chinese Academy of Sciences,Beijing 100049;2. Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016)
  • Received:2015-01-11 Online:2015-09-15 Published:2015-09-16

Abstract: This work is to analyze and compare the superantigen sensitivity on staphylococcal enterotoxins C2(SEC2)in guinea pigs and BALB/c mice, and determine the model animal for the researches of superantigen sensitivity on SEC2. In vitro, peripheral blood mononuclear cell(PBMC)and splenocytes from guinea pigs and BALB/c mice were respectively stimulated with SEC2 at gradient concentration, and the cell proliferation was tested by MTS assay;In vivo, the changes of temperature and weight of guinea pigs and mice were measured at interval of 3 d after the intraperitoneal injection of SEC2. Meanwhile, the serums were collected at interval of 7 d to detect the levels of IL-4, IFN-γ, IL-2 and TNF-α by ELISA. SEC2 of different concentrations significantly stimulated the proliferation of PBMC and splenocytes from BALB/c mouse(P < 0.05), but not so from guinea pigs(P > 0.05). After intraperitoneal injection of SEC2, there was no significant difference in body temperature of both guinea pigs and mice(P > 0.05)compared to the control. In the later phase of the experiment, the body weights of guinea pigs in SEC2 group were significantly lower than those in control group(P < 0.05). And the body weights of mice in SEC2 group were also significantly lower than those in control group during day 3 to 30, but recovered to normal level at the end of the experiment(P > 0.05). The cytokines IL-4, IFN-γ, IL-2 and TNF-α in serum of mice of SEC2 group were significantly elevated compared with in control group(P < 0.05). However, there was no significant difference in guinea pigs administrated with SEC2 and physiological saline(P > 0.05). In conclusion, comparing with guinea pigs, BALB/c mice are more sensitive to the stimulation of SEC2 with promising dose-dependent and time-dependent manner. Therefore, BALB/c mouse is a suitable model animal for the researches of superantigen activity.

Key words: guinea pig, mouse, staphylococcal enterotoxins C2