Research Advances on Molecular Techniques for Improving Microbial Tolerance to Organic Solvents

doi:10.13560/j.cnki.biotech.bull.1985.2015.10.015

Abstract

Abstract: Organic-solvent-tolerant microorganisms can survive and grow in the higher concentrations of organic solvents, which exhibit significant application advantages in the field of the non-aqueous phase biocatalysis. Compared to natural screening, long-term domestication and traditional mutagenesis methods, molecular technique is a more rational and efficient way to obtain organic-solvent-tolerant strains. This paper mainly summarizes the research advances on the improvement of microbial organic-solvent tolerance by the reformation of functional genes related to the resistance to organic solvents and transcriptional factors using molecular biology techniques, also prospects the application of organic-solvent-tolerant strain.

Key words: organic solvent tolerance, molecular biology techniques, engineered strain, biotransformation

Wang Yanxia, Liu Xiangsheng, Wang Min, Luo Jianmei. Research Advances on Molecular Techniques for Improving Microbial Tolerance to Organic Solvents[J]. Biotechnology Bulletin, 2015, 31(10): 77-88.

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