A Study on OpZFP Increasing Salt Tolerance of Nicotiana tabacum

doi:10.13560/j.cnki.biotech.bull.1985.2015.10.020

Abstract

Abstract: To research the role of C2H2 zinc finger protein in signal transduction under abiotic stresses and the developmental processes of plant, an OpZFP was cloned from Olimarabidopsis pumila in Xinjiang. An OpZFP was the single C2H2 gene cloned from Olimarabidopsis pumila in Xinjiang. Analysis of semi-quantitative RT-PCR indicated that OpZFP was successfully expressed in the transgenic tobacco. Interestingly, the overexpressed transgenic plants showed more tolerance to salt stress compared with the wild plants. Under high salt stress, the root lengths of transgenic plants were longer, and they contained lower MDA contents than the wild type. Further experiments revealed that the transgenic plants had a lower degree of bleaching than the wide type ones. These results indicated that the OpZFP-overexpressing transgenic plants could enhance plant resistance to salt stress.

Key words: Olimarabidopsis pumila, zinc finger protein, salt tolerance, Nicotiana tabacum

Wang Qiqi, Guo Danli, Wu Xiaoqing, Fei Chunyan, Huang Xianzhong. A Study on OpZFP Increasing Salt Tolerance of Nicotiana tabacum[J]. Biotechnology Bulletin, 2015, 31(10): 119-124.

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