Preparation of Drought-resistant and Salt-tolerant Bacteria and Its Effect on Germination of Licorice Seeds

doi:10.13560/j.cnki.biotech.bull.1985.2020-0120

Abstract

Abstract: The objective of this work is to prepare the highly effective stress-resistant microbial agent and to study their effects on the growth,development and physiological and biochemical characteristics of licorice under drought and salt stress. Single factor and orthogonal experiments were used to optimize the fermentation medium of Bacillus pumilus. Single factor experiments were combined with the Box-Behnken test to optimize the fermentation process,and a highly effective stress-resistant microbial agent was obtained. The hydroponic germination test was used to verify its effects on licorice growth under drought and salt stress and its mechanism. Results showed that the optimized fermentation medium was as：maltose 4.0%,soy peptone 3.0%,KH₂PO₄ 0.01%,and the rest was water. The optimized fermentation process was as：fermentation time 42 h,fermentation temperature 34℃,initial pH 7.00,shaking speed 230 r/min,the inoculation amount 2.0%,and the liquid content 100 mL/250 mL. The germination and growth indexes of licorice seedlings under drought and salt stress were improved to varying degrees. The inoculated agent significantly increased the contents of antioxidants（glutathione and ascorbate）and osmotic regulators（soluble sugars and soluble proteins）. Inoculation with bacterial agents under drought and salt stress significantly increased the activities of carbon and nitrogen metabolism enzymes. The activity of nitrate reductase increased by 77% under drought stress,and the activity of sucrose invertase increased by 445.5% under salt stress. In conclusion,the B. pumilus obtained in this study may improve the drought resistance and salt tolerance of licorice by increasing the content of antioxidants,osmoregulatory substances and regulating the activities of carbon and nitrogen metabolism enzymes,thereby promote its growth.

Key words: microbial agents, drought and salt tolerance, antioxidant, osmotic adjustment, carbon and nitrogen metabolism

ZHANG Xiao-jia, LU Ya-jun, ZHANG Wen-jin, ZHANG Yu, CUI Gao-chang, LANG Duo-yong, ZHANG Xin-hui. Preparation of Drought-resistant and Salt-tolerant Bacteria and Its Effect on Germination of Licorice Seeds[J]. Biotechnology Bulletin, 2020, 36(9): 180-193.

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