Abstract: This experiment was devoted to screen the strains of high-yield lipase, identify the genera, and define the optimal fermentation conditions. The strains were preliminary screened from greasy soil nearby Lanzhou urban area through Victoria blue and rhodamine B plates. The strains were re-screened through a copper soap spectrophotography measuring lipase activity while using emulsified olive oil as the substrate, finally a fungal strain LZ-6 with high-yield lipase was obtained. It was identified as Actinomucor elegans through morphological observation and molecular identification. The fermentation conditions for lipase production optimized by the single factor experiments and the orthogonal experiments with strain LZ-6 were：yeast powder 1%, olive oil 2%, 0.05% MgSO₄·7H₂O, 0.1%（NH₄）₂SO₄, 0.2% K₂HPO₄, fermentation temperature at 30℃, pH5.0, 2% inoculums volume, and fermentation time of 5 days; under the optimal condition, the lipase activity of strain LZ-6 reached 13.35 U/mL, it increased 1.78 times as before optimization.

Key words: fungi strain, lipase, screening, identification, optimization