Identification of Endophytic Fungi in Bupleurum and Screening of Antimicrobial Activities

doi:10.13560/j.cnki.biotech.bull.1985.2015.10.026

Abstract

Abstract: In order to determine the main distribution of endophytes and their broad-spectrum antimicrobial activity, 27 strains of endophytic fungi were isolated from the stems, roots and leaves of wild bupleurum at the Ganzi Tibetan Autonomous Prefecture of Sichuan Province. Identification of these strains was determined by their morphological features. They were classified as 9 fungal genera in which Aspergillus and Penicillium were the dominant. The antimicrobial activities from endophytic fungi were investigated by using the agar diffusion method and the cylinder plate method, and Bacillus subtilis, Escherichia coli and Staphylococcus aureus as indicator microorganisms. The results showed that 26 strains, accounting for 96.30% of all the isolates, had antimicrobial activities to 2 indicators or 3. Endophytic fungi of CHG-01, CHG-04, CHJ-10, CHY-01, and CHY-03 had remarkable antimicrobial activities to 3 indicators respectively. There is natural antimicrobial substance in the secondary metabolites of endophytic fungi from the wild bupleurum, which provides a new source for searching novel antimicrobial products.

Key words: endophytic fungi, antimicrobial activity, bupleurum

Li Lingling, Luo Hechun, Zhang Xianshu. Identification of Endophytic Fungi in Bupleurum and Screening of Antimicrobial Activities[J]. Biotechnology Bulletin, 2015, 31(10): 165-170.

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