The Differential Expression Characteristics of miR319 and Four Targets Response to Low Temperature Treatments in Two Cassava Varieties

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.015

Abstract

Abstract: To study the regulation roles of microRNAs in cassava low temperature adaption, our early small RNA transcriptome profile identified miR319 response to low temperature in cassava. This study, we assayed the expression patterns of miR319 and its 4 different target genes between two cassava varieties under 4 low temperature treatments based on real-time quantitative polymerase chain reaction(PCR)technique. The results showed that the expression patterns of miR319a and its target genes differed in two different cassava varieties, differed among the four cryogenic treatments, and same to the correlation coefficients between microRNAs and four different target genes. After the comparison study, we found that the negative correlations between miR319 with three targets(MYB33, TCP4 and Unknown)are larger in C4 than those in SC124, and the NAH treatment has most typical negative correlation, and the order of negative correlation coefficients between miR319 and its target gene from large to small are: MYB33>Unknown>TCP4>GSTU8. All those data reveal that MYB33 and Unknown target(021030m)are priority target genes miR319 might execute its negative regulation under NAH treatment in C4, and thus the regulation of miR319 on MYB33 and unknown target in cassava low temperature adoption is worth in-depth study in the future.

Key words: miR319, chilling tolerance, targets, negative regulation, correlation coefficients

Zeng Changying, Zhou Yufei, Peng Ming. The Differential Expression Characteristics of miR319 and Four Targets Response to Low Temperature Treatments in Two Cassava Varieties[J]. Biotechnology Bulletin, 2015, 31(11): 173-178.

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