Effect of Exogenous Abscisic Acid on Chilling Tolerance and Glycine Betaine Accumulation in Jatropha curcas Seedlings Under Chilling Stress

doi:10.13560/j.cnki.biotech.bull.1985.2016.09.014

Abstract

Abstract: The objective is to investigate the effect of exogenous abscisic acid（ABA）on chilling tolerance and glycine betaine accumulation of Jatropha curcas seedlings under chilling stress. Seedlings of J. curcas under chilling stress were treated with 150 μmol/LABA,and the survival rate,root vitality,electrolyte leakage,the content of malondialdehyde（MDA）,the content of glycine betaine,and the activity of the key enzyme betaine aldehyde dehydrogenase（BADH）of glycinebataine biosynthesis as well as the expression of JcBADH were measured. The results showed that treatment with 150 μmol/L ABA significantly increased survival rate and root vitality,decreased MDA content and electrolyte leakage in J. curcas seedlings under chilling stress. ABA also enhanced the accumulation of glycine betaine,increased the activity of BADH,and improved the expression level of the JcBADH. The results suggested that exogenous ABA treatment may enhance chilling tolerance in J. curcas seedlings,and glycine betaine might play a key role in the increasing of chilling tolerance.

Key words: abscisic acid, chilling stress, glycine betaine, chilling tolerance, Jatropha curcas

YANG Shuang-long, DENG Feng-fei, DU Chao-kun, CHENG Yun-xiu, GONG Ming. Effect of Exogenous Abscisic Acid on Chilling Tolerance and Glycine Betaine Accumulation in Jatropha curcas Seedlings Under Chilling Stress[J]. Biotechnology Bulletin, 2016, 32(9): 100-106.

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