Optimization of Bacillus sublitis JH-1 for Xylanase Production by Response Surface Analysis

doi:10.13560/j.cnki.biotech.bull.1985.2015.02.027

Abstract

Abstract: Xylanase producing strain Bacillus sublitis JH-1 was screened from straw compost by Congo red transparent circlemethod. According to the single-factor experiments, response surface methodology was applied to optimize the liquid state fermentation conditions of Bacillus sublitis JH-1 for xylanase production, with the optimal conditions being listed as follows：temperature as 33℃, pH value as 6.0, maltose as 2.6%（m/V）, YE as 1.2%（m/V）, KH₂PO₄ as 1.2%（m/V）. Increased 1.8 times in average as compared with preliminary culture and consistent with the maxmum predicted value.

Key words: Bacillus sublitis JH-1, xylanase, Response Surface Methodology

Wang Chunming, Zhong Chao, Wang Fengxue, Huang Fan, Jia Honghua, Wei Ping, Zhao Yin. Optimization of Bacillus sublitis JH-1 for Xylanase Production by Response Surface Analysis[J]. Biotechnology Bulletin, 2015, 31(2): 179-186.

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