The Factors Affecting Genetic Transformation of Soybean Cotyledon Node Mediated by Agrobacterium tumefacions

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.013

Abstract

Abstract: It was to improve the efficiency of soybean genetic transformation, we used the soybean cotyledon node as explants via Agrobactium-mediated transformation method, and investigated the effect of bacterial concentration, co-culture temperature and time on the transformation efficiency and the rate of bud induction. The results showed that under the OD₆₀₀= 0.5 of infection solution concentration, and induction rate was the highest. The conversion efficiency was higher under the environment of co-culture temperature being 24℃ for 10 days. The single-wall carbon nanotubes added to culture medium improved the induction of the adventitious bud with kanamycin-resistance. Moreover, the PCR results indicated that PHR1 gene was integrated into T1 genome generation, preliminarily proving that the heredity of gene in the soybean genome was stable.

Key words: Agrobactium-mediated, soybean cotyledon node, co-culture, single-wall carbon nanotube

Yang Xiaoqian, Li Guilan, Liu Chenguang, Dong Qiuping, Zhang Kai, Qiao Yake. The Factors Affecting Genetic Transformation of Soybean Cotyledon Node Mediated by Agrobacterium tumefacions[J]. Biotechnology Bulletin, 2015, 31(12): 91-96.

References

[1]余永亮, 梁慧珍, 王树峰, 等.中国转基因大豆的研究进展及其产业化[J].大豆科学, 2010, 29(1):143-150.
[2]任海祥, 南海洋, 曹东, 等. 大豆转基因技术研究进展[J]. 东北农业大学学报, 2012, 43(7):6-12.
[3]Somers DA, Samac DA, Olhoft PM. Recent advances in legume transformation[J]. Plant Physiology, 2003, 131:892-899.
[4]刘圣君, 黄健秋, 卫志明.影响农杆菌介导的大豆子叶节遗传转化的因素[J].分子细胞生物学报, 2007, 40(5):286-292.
[5]刘翠, 李喜焕, 常文锁, 等.农杆菌介导大豆不同外植体遗传转化研究]J].华北农学报, 2012, 27(3):35-40.
[6]Bertolla F, Kay E, Simonet P. Potential dissemination of antibiotic restistace genes from transgenic plants to microorganisms[J]. Infect Control Hosp Epidemiol, 2000, 21(6):390-393.
[7]Losey JE, Rayor LS, Carter ME. Transgenic pollen harms monarch larvae[J]. Nature, 1999, 399:214-215.
[8]祁永斌, 刘庆龙, 陆艳婷, 等.转基因植物中删除选择标记基因的研究进展[J].浙江农业学报, 2014, 26(5):1387-1393.
[9]周思军, 李希臣, 刘昭军, 等. 大豆农杆菌介导转化系统的优化研究[J]. 大豆科学, 2001, 32(4):313-319.
[10]武小霞, 李静, 刘伟婷, 等. 大豆农杆菌子叶节转化菌株适宜生长时期及浸染浓度的研究[J]. 东北农业大学学报, 2010, 41(1):1-6.
[11]刘海坤卫志明. 利用农杆菌介导转化大豆成熟种子胚尖获得转基因植株[J[. 植物生理与分子生物学学报, 2004, 30(6):631-636.
[12]段莹莹, 赵琳, 陈李淼, 等.农杆菌介导的大豆子叶节和下胚轴转化方法的比较及优化[J].大豆科学, 2010, 29(4):590-593.
[13]Meurer CA, Dinkins RD, Collins GB. Factors affecting soybean cotyledonary node transformation[J]. Plant Cell Rap, 1988, 18:180-186
[14]应珊, 何晓薇, 王秀荣, 寿惠霞.影响农杆菌介导的大豆转化效率的因素研究[J].分子植物育种, 2008, 6(1):32-40.
[15]Masciangioli T, Zhang WX. Environmental technologies at the nanoscale[J]. Environ Sci Technol, 2003, 37:102a-108a.
[16]吕继涛, 张淑贞. 人工纳米材料与植物的相互作用:植物毒性、吸收与传输[J]. 化学进展, 2013, 25(1):156-163.
[17]陆长梅, 张超英, 温俊强, 等. 纳米材料促进大豆萌发, 生长的影响及其机理研究[J]. 大豆科学, 2002, 21:168-172.
[18]Ma LL, Liu C, Qu CX, et al. Rubisco activase mRNA expression in spinach:Modulation by nanoanatase treatment[J]. Biological Trace Element Research, 2008, 122(2):168-178.
[19]Khodakovskaya M, Dervishi E, Mahmood M, et al. Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth[J]. American Chemical Society Nano, 2009, 10:3221-3227.
[20]王晓红. 单壁碳纳米管对大豆子叶节外植体分化与再生影响的研究[D]. 北京:中国农业科学院, 2013.
[21]杨致荣, 王兴春, 李西明.高等植物转录因子的研究进展[J]. 遗传, 2004, 26(3):403-408.
[22]张彩英, 李喜焕, 常文锁, 等.大豆 GmPHR1 基因及其编码的蛋白和应用:中国, ZL 201010528355.X[P].2012-09-05.