Construction of Tetracycline-degrading Bacterial Co-culture System and Community Analysis of Wastewater Remediation

doi:10.13560/j.cnki.biotech.bull.1985.2020-0232

Abstract

Abstract:

Tetracycline-containing wastewater widely exists in environment,and it has attracted much attention due to issues such as resistance gene transmission,human drug resistance,etc. After comparing the ability of bacteria degrading tetracycline,the co-culture system mainly with strain Raoultella sp. XY-1 and strain Pandoraea sp. XY-2 was clearly constructed. Then this system was applied in actual wastewater for bioremediation,and the removal efficiency of pollutants and bacterial community changes were recorded. The results showed that degradation percentage by this co-culture system for 10 d was > 80% in glucose medium with 80 mg/L tetracycline. The co-culture system presents a favorable effect on the removal of pollutants from actual tetracycline-containing wastewater,and the results by high-through sequencing community indicated that the strain Raoultella sp. XY-1 became the dominant bacterium.

Key words: tetracycline wastewater, bioremediation, microbial co-culture, bacteria community

WU Xue-ling, ZHOU Xiang-yu, WU Xiao-yan, LUO Kui, GU Yi-chao, ZHOU Han, LIAO Wan-qing, ZENG Wei-min. Construction of Tetracycline-degrading Bacterial Co-culture System and Community Analysis of Wastewater Remediation[J]. Biotechnology Bulletin, 2020, 36(10): 116-126.

Figures/Tables 7

References 37

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添加浓度/ （mg?L^-1）	回收率/100%			${x}^{-}±SD \%$	CV/100%
添加浓度/ （mg?L^-1）	1	2	3	${x}^{-}±SD \%$	CV/100%
10	87.01	88.23	89.06	88.14±0.84	0.95
40	86.04	87.68	89.52	87.75±1.42	1.61

添加浓度/ （mg?L^-1）	回收率/100%			${x}^{-}±SD \%$	CV/100%
添加浓度/ （mg?L^-1）	1	2	3	${x}^{-}±SD \%$	CV/100%
10	87.01	88.23	89.06	88.14±0.84	0.95
40	86.04	87.68	89.52	87.75±1.42	1.61

样本	对照组	Raoultella sp. XY-1	Pandoraea sp. XY-2	共培养系统	时间/d	显著性
四环素浓度	80.83±1.05a	80.38±0.55a	81.77±1.59a	79.77±0.70a	0	0.112
四环素浓度	65.90±0.74a	19.1±0.07c	28.94±0.32b	13.91±0.08d	12	1.000

样本	对照组	Raoultella sp. XY-1	Pandoraea sp. XY-2	共培养系统	时间/d	显著性
四环素浓度	80.83±1.05a	80.38±0.55a	81.77±1.59a	79.77±0.70a	0	0.112
四环素浓度	65.90±0.74a	19.1±0.07c	28.94±0.32b	13.91±0.08d	12	1.000

检测项目	空白	对照组	实验组	检测项目	空白	对照组	实验组
四环素浓度	3.34278	2.23645	1.03205	pH	6.82	7.04	8.31
COD_Cr	462.16	386.56	224.65		NH₄⁺-N	3.92	3.66	2.03
总氮	27.45	23.22	12.33		NO₃^--N	18.32	12.64	4.96
Ag	<0.001	<0.001	<0.001		As	0.031	0.011	<0.005
Cd	0.006	0.002	<0.001		Co	0.015	0.004	<0.001
Cr	0.013	0.009	<0.002		Cu	0.311	0.201	0.152
Fe	0.587	0.392	<0.005		Hg	0.007	0.003	0.003
Mn	<0.001	<0.001	<0.001		Ni	0.015	0.006	0.003
Pb	<0.009	<0.009	<0.009		Zn	0.321	0.154	<0.001