The Microwave-assisted Two-phase Solvent Extraction of Essential Oil from Taiwanofungus camphoratus and Its Antifungal Activity

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.018

Abstract

Abstract: In this study microwave-assisted petroleum ether/ethanol two-phase solvent extraction was applied to extract the essential oil from the fermentation broth of Taiwanofungus camphoratus, and the minimal inhibitory concentration(MIC)of the essential oil against 7 species of dermatophyte were determined. The optimized extraction conditions are as following:ethanol concentration 56%(v/v), petroleum ether concentration 30%(v/v), solid-to-liquid ratio 1:50, microwave power 380 W and microwave time 90 s. The highest yield of extracted essential oil was 0.69%, and the MIC against the tested fungi were 5-20 mL/L. This extraction technology is efficient and time saving, moreover, the produced essential oil shows significant inhibitory effect on the growth of the tested dermatophyte.

Key words: microwave-assisted two-phase solvent extraction, essential oil, Taiwanofungus camphoratus, minimal inhibitory concentration(MIC), dermatophyte

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Liu Lin, Chen Huixia, Guo Lizhong. The Microwave-assisted Two-phase Solvent Extraction of Essential Oil from Taiwanofungus camphoratus and Its Antifungal Activity[J]. Biotechnology Bulletin, 2015, 31(12): 122-130.

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https://biotech.aiijournal.com/EN/Y2015/V31/I12/122

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The Microwave-assisted Two-phase Solvent Extraction of Essential Oil from Taiwanofungus camphoratus and Its Antifungal Activity

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[1]	HUANG Ya-ning, ZHANG Hai-jiao, HAN Yu-qian, LIU Zun-ying. Effects of Supercritical CO₂ Combined with Ginger Essential Oil on the Sterilization of Vibrio parahaemolyticus and Its Mechanism [J]. Biotechnology Bulletin, 2023, 39(5): 297-305.
[2]	HU Jian-ran, LI Ping, TIE Jun, JIN Shan. Study on Antioxidant and Antitumor Activity of Essential Oil from Flowers of Syringa oblata [J]. Biotechnology Bulletin, 2019, 35(12): 16-23.
[3]	YAN Kuan, CHEN Fang, WEI Qin, FENG Rui-zhang, ZHOU Wan-hai, ZHOU Min. Effect of Endophytic Fungi on Iits Essential Oil Accumulation and Physiological-biochemical Characteristic of Cinnamomum longepaniculatum [J]. Biotechnology Bulletin, 2017, 33(3): 138-143.
[4]	Nie Ying, Li Shuying, Qi Xiaoyu, Yang Fan, Mu Taihua, Tang Xuanming. Preliminary Research for Inhibitory Effect of Essential Oil from Cuminum cyminum Against Pathogenic Microorganisms on Fruit and Vegetable Storage [J]. Biotechnology Bulletin, 2013, 0(4): 167-171.