Effect of Endophytic Fungi on Iits Essential Oil Accumulation and Physiological-biochemical Characteristic of Cinnamomum longepaniculatum

doi:10.13560/j.cnki.biotech.bull.1985.2017.03.020

Abstract

Abstract: The aim of this study was to examine the effects of endophytic fungi on the accumulation and physiological-biochemical characteristics of essential oil in Cinnamomum longepaniculatum. The essential oil of C. longepaniculatum was extracted after spraying four endophytic fungi spore suspension（2J1、3J1、5J2、YB）on the leaves separately,and the content of 1,8-cineole and α-terpineol was determined by GC-MS. The results showed that the content of essential oil reached a peak at 21 d after treatment with 2J1 and 3J1,and the contents of 1,8-cineole increased by 201.53% and 174.47% compared with controls,and α-terpineol increased by 364.14% and 307.29% as well,respectively. Furthermore,the peroxidase（POD）content,catalase（CAT）activity,and malondialdehyde（MOD）content of suspension cells increased significantly. No significant difference was detected between C. longepaniculatum treated with 5J2 and the control group in terms of essential oil accumulation,CAT and POD activity,and MOD contents,nor did YB. Consequently,treatment with 2J1 and 3J1 could significantly increase the accumulation of essential oil in C. longepaniculatum.

Key words: endophytic fungi, Cinnamomum longepaniculatum, essential oil production, physiological- biochemical characteristic

YAN Kuan, CHEN Fang, WEI Qin, FENG Rui-zhang, ZHOU Wan-hai, ZHOU Min. Effect of Endophytic Fungi on Iits Essential Oil Accumulation and Physiological-biochemical Characteristic of Cinnamomum longepaniculatum[J]. Biotechnology Bulletin, 2017, 33(3): 138-143.

References

[1] 陶光复, 丁靖垲, 孙汉董. 湖北油樟叶精油的化学成分[J]. 武汉植物学研究, 2002, 20（2）：75-77.
[2] 王坚, 罗永明, 刘贤旺. 樟科樟属植物的化学成分与组织培养研究[J]. 江西中医学院学报, 2004, 16（2）：69-70.
[3] Xu S, Yin ZQ, Wei Q, et al. Anti-hepatoma effect of safrole from Cinnamomum longepaniculatum leaf essential oil in vitro[J]. Int J Clin Exp Pathol, 2014, 7：2265-2272.
[4] 王涛, 魏淑芳, 魏琴, 油樟叶内生真菌的多样性研究[J]. 云南大学学报：自然科学版, 2007, 29（3）：300-302.
[5] Hyde K, Frohlich J, Taylor J. Diversity of ascomycetes on palms in the tropics[M]. Hong Kong University Press, 1997：141-156.
[6] Zhuang T, Cheng L. Soil microbial function of Kandelia candel mangrove：degradation of diesel oil[A]. Hongkong university Press, 1998：389-395.
[7] 林燕青, 洪伟. 植物内生真菌研究及应用前景[J]. 福建林业科技, 2012, 39（3）：186-193.
[8] Spiering MJ, Lane GA, Christensen MJ, et al. Distribution of the fungal endophyte Neotyphodium lolii is not a major determinant of the distribution of fungal alkaloids in Lolium perenne plants[J]. Phytochemistry, 2005, 66（2）：195-202.
[9] Chandra S. Endophytic fungi：novel sources of anticancer lead molecules[J]. Applied Microbiology and Biotechnology, 2012, 95（1）：47-59.
[10] Hussain H, Krohn K. Bioactive chemical constituents of two endophytic fungi[J]. Biochem Systemat Ecol, 2007, 35：898-900.
[11] Phongpaichitl S, Nikom J. Biological activities of extracts from endophytic fungi isolated from Garcinia plants[J]. FEMS Immunol Med Microbiol, 2007, 51：517-525.
[12] Kuklinsky-Sobral J, Araújo WL, Mendes R, et al. Isolation and characterization of soybean-associated bacteria and their potential for plant growth promotion[J]. Environmental Microbiology, 2004, 6（12）：1244-1251.
[13] Kuldau G, Bacon C. Clavicipitaceous endophytes：their ability to enhance resistance of grasses to multiple stresses[J]. Biological Control, 2008, 46：57-71.
[14] Kusari S, Hertweck C, Spiteller M. Chemical ecology of endophytic fungi：origins of secondary metabolites[J]. Chemistry and Biology, 2012, 19（7）：792-798.
[15] 姚祥, 李秀璋, 朱小晓, 等. 两种杀菌剂对中华羊茅种传内生真菌的影响[J]. 草业学报, 2013, 30（10）：1517-1522.
[16] Aly A H, Debbab A, Proksch P. Fungal endophytes-secret producers of bioactive plant metabolites[J]. Pharmazie, 2013, 68（7）：499-505.
[17] Photital W, Hyde KD. Endophytic fungi of wild banana（Musa acuminata）at DoiSuthep Pui National Park, Thailand[J]. Mycological Research, 2001, 105（12）：1508-1513.
[18] 杜素娟, 郭晓恒. 植物内生真菌对植物次生代谢产物的影响[J]. 现代农业科学, 2009, 16（5）：17-19.
[19] 刘吉华, 余伯阳. 喜树内生真菌的分离及其抗肿瘤活性代谢产物的筛选方法[J]. 植物资源与环境学报, 2004, 13（4）：6-10.
[20] 赵鸿莲, 于荣敏. 诱导子在植物细胞培养中的应用研究进展[J]. 沈阳药科大学学报, 2000, 17（2）：152-155.
[21] 陶金华, 濮雪莲, 江曙. 内生真菌诱导子对茅苍术细胞生长及苍术素积累的影响[J]. 中国中药杂志, 2011, 36（1）：27-31.