Sequence Analysis and Prokaryotic Expression of GmGS1γ Gene from Nodules of Glycine soja

doi:10.13560/j.cnki.biotech.bull.1985.2016.04.015

Abstract

Abstract: In order to clarify the structures and functions of the members of the glutamine synthetase（GS）gene family，the GmGS1 gene was cloned from Glycine soja by homologous cloning. The bioinformatics analysis showed that，the ORF was 1 071 bp，and the similarity was 100% and 99% with the partial sequence of soybean GS1γ（AF363022.1）and X81700.1，respectively. The sequence had two conserved domains of plant GS，beta-Grasp GS functional area（17-97 aa），and GS catalytic functional area（103-350 aa）. Phylogenetic tree showed that GS encoded by the gene GmGS1 probably belonged to glutamine synthetase cytosolic isozyme 2. The gene was expressed in Escherichia coli DE3.0，and the molecular weight of the protein was 44 kD.

Key words: Glycine soja, glutamine synthetase（GS）, prokaryotic expression, sequence analysis

YANG Mei-ying, YUE Sheng-tian ,HAN Hong ,SUN He-mei, LIU Jing-jing, LU Dong-xue. Sequence Analysis and Prokaryotic Expression of GmGS1γ Gene from Nodules of Glycine soja[J]. Biotechnology Bulletin, 2016, 32(4): 116-120.

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