Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (12): 151-159.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0368
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ZHANG Hao1,2(), ZHANG Ya-nan1,2, LI Xin1,2, WANG Jia-mei1,2, WANG Yong1(), ZHU Jiang-jiang1, XIONG Yan1,2, LIN Ya-qiu1,2()
Received:
2021-03-22
Online:
2021-12-26
Published:
2022-01-19
Contact:
WANG Yong,LIN Ya-qiu
E-mail:zhanghao9235@163.com;wangyong010101@swun.cn;linyq1999@163.com
ZHANG Hao, ZHANG Ya-nan, LI Xin, WANG Jia-mei, WANG Yong, ZHU Jiang-jiang, XIONG Yan, LIN Ya-qiu. Effect of PDK4 on the Lipid Metabolism of Goat Intramuscular Adipocytes[J]. Biotechnology Bulletin, 2021, 37(12): 151-159.
基因 Gene | 登录号 GenBank | 退火温度 Annealing temperature/℃ | 序列 Primer sequence(5'-3') | 产物长度 Product length/bp | 用途 Purpose |
---|---|---|---|---|---|
PDK4 | NM_001101883.1 | 60 | S:GAGTCTCAAGCCACCTACGG | 1 806 | PCR |
A:TGACATCAATCAGTGGCCTAA | |||||
PDK4 | MF564045.1 | 53 | S:ACCTCTTTTGCATTTTTGCG | 111 | qPCR |
A:TTTGGTCCTCTGGGCTTTTC | |||||
TBP | XM_018053502.1 | 60 | S:AACAGCCTCCCACCTTATGC | 155 | qPCR |
A:TGCTGCTCCTCCAAAATAGAC | |||||
CD36 | X91503 | 60 | S:GTACAGATGCAGCCTCATTTCC | 81 | qPCR |
A:TGGACCTGCAAATATCAGAGGA | |||||
FABP3 | NM_174313.2 | 58 | S:GATGAGACCACGGCAGATG | 120 | qPCR |
A:GTCAACTATTTCCCGCACAAG | |||||
AGPAT6 | JI861797.1 XM_018041984.1 | 60 | S:AAGCAAGTTGCCCATCCTCA | 101 | qPCR |
A:AAACTGTGGCTCCAATTTCGA | |||||
ADRP | HQ846827 NM_001285596.1 | 60 | S:TACGATGATACAGATGAATCCCAC | 202 | qPCR |
A:CAGCATTGCGAAGCACAGAGT | |||||
ATGL | GQ918145 | 60 | S:GGAGCTTATCCAGGCCAATG | 180 | qPCR |
A:TGCGGGCAGATGTCACTCT | |||||
UXT | NM_001037471 | 60 | S:CAGCTGGCCAAATACCTTCAA | 125 | qPCR |
A:GTGTCTGGGACCACTGTGTCAA |
Table 1 Primer information
基因 Gene | 登录号 GenBank | 退火温度 Annealing temperature/℃ | 序列 Primer sequence(5'-3') | 产物长度 Product length/bp | 用途 Purpose |
---|---|---|---|---|---|
PDK4 | NM_001101883.1 | 60 | S:GAGTCTCAAGCCACCTACGG | 1 806 | PCR |
A:TGACATCAATCAGTGGCCTAA | |||||
PDK4 | MF564045.1 | 53 | S:ACCTCTTTTGCATTTTTGCG | 111 | qPCR |
A:TTTGGTCCTCTGGGCTTTTC | |||||
TBP | XM_018053502.1 | 60 | S:AACAGCCTCCCACCTTATGC | 155 | qPCR |
A:TGCTGCTCCTCCAAAATAGAC | |||||
CD36 | X91503 | 60 | S:GTACAGATGCAGCCTCATTTCC | 81 | qPCR |
A:TGGACCTGCAAATATCAGAGGA | |||||
FABP3 | NM_174313.2 | 58 | S:GATGAGACCACGGCAGATG | 120 | qPCR |
A:GTCAACTATTTCCCGCACAAG | |||||
AGPAT6 | JI861797.1 XM_018041984.1 | 60 | S:AAGCAAGTTGCCCATCCTCA | 101 | qPCR |
A:AAACTGTGGCTCCAATTTCGA | |||||
ADRP | HQ846827 NM_001285596.1 | 60 | S:TACGATGATACAGATGAATCCCAC | 202 | qPCR |
A:CAGCATTGCGAAGCACAGAGT | |||||
ATGL | GQ918145 | 60 | S:GGAGCTTATCCAGGCCAATG | 180 | qPCR |
A:TGCGGGCAGATGTCACTCT | |||||
UXT | NM_001037471 | 60 | S:CAGCTGGCCAAATACCTTCAA | 125 | qPCR |
A:GTGTCTGGGACCACTGTGTCAA |
Fig. 1 Sequence analysis of goat PDK4 A:Information of PDK4 sequence and coding amino acid. B:Prediction of PDK4 domain. C:Prediction of PDK4 secondary structure. D:Prediction of PDK4 interaction protein
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