Cloning,Expression and Anticancer Mechanism of Scolopin 2,an Antimicrobial Peptide from Centipede Venoms（Scolopendra subspinipes mutilans）

doi:10.13560/j.cnki.biotech.bull.1985.2016.09.023

Abstract

Abstract: This work is to explore the antimicrobial activity and anticancer mechanism of AMP-scolopin 2. The small ubiquitin-related modifier（SUMO）fusion technology was applied in the expression of AMP-scolopin 2 in vitro. The technologies of inhibition zone assay,MTT,cytotoxicity assay,flow cytometry and Western blotting were utilized to analyze its antimicrobial and hemolytic activity as well as apoptotic effects on cancer cells. As results,AMP-scolopin 2 was successfully expressed and purified. The AMP-scolopin 2 inhibited the proliferation of K562 leukemia cells and HepG2 hepatocellular carcinoma,while it had only a minimal effect on erythrocytes and normal HEK293 cells. AMP-scolopin 2（40 μmol/L）induced the apoptosis of 21.4% K562 leukemia cells and 18.5% HepG2 hepatocellular carcinoma respectively. In addition,AMP-scolopin 2 down-regulated the expression level of apoptosis proteins（pro-caspase-3,-9 and pro-PARP）that were related to mitochondria in a dose-dependent mode. In summary,the present study indicates that AMP-scolopin 2 promotes the apoptosis of tumor cells in vitro through mitochondrial caspase-dependent pathway.

Key words: centipede, AMP-scolopin 2, SUMO, antimicrobial activity, apoptosis

HOU Huan-huan, LU Jia, ZHANG Wei-jing, HUANG Fang, REN Wen-hua. Cloning,Expression and Anticancer Mechanism of Scolopin 2,an Antimicrobial Peptide from Centipede Venoms（Scolopendra subspinipes mutilans）[J]. Biotechnology Bulletin, 2016, 32(9): 172-178.

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