Review on Effects of Disulfide Bond and Its Connection on Antimicrobial Activity in Defensins

doi:10.13560/j.cnki.biotech.bull.1985.2016.09.005

Abstract

Abstract: Defensins are a class of endogenous,highly stable and cysteine-rich antimicrobial peptides,which play an important role in anti-microbial infection and the regulation of host immune. Six to eight conserved cysteines in the sequence form 3 to 4 pairs of disulfide bonds. The number of disulfide bond and its connection play an crucial role in maintaining the structure and antibacterial functions of defensins. In addition,mutants with high activity and low disulfide bond content also play an key role in reducing production costs and simplifying the production processes. The molecular characteristics,structure and function of defensins and their effects of redox status,number and connection mode of disulfide bonds on the antimicrobial activity are reviewed.

Key words: defensin, disulfide bond, oxidation and reduction, linkage, antimicrobial activity

CHEN Hui-xian^{1, 2, 3}, MAO Ruo-yu^{1, 3}, TENG Da^{1, 3}, WANG Xiu-min^{1, 3}, FENG Xing-jun², WANG Jian-hua^{1, 3}. Review on Effects of Disulfide Bond and Its Connection on Antimicrobial Activity in Defensins[J]. Biotechnology Bulletin, 2016, 32(9): 32-37.

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