Study of Plasma Differential Proteins in fat-1 Transgenic Cow

doi:10.13560/j.cnki.biotech.bull.1985.2016.09.027

Abstract

Abstract: Using the plasma proteins in fat-1 transgenic cow as the research material,the potential mechanism of fat-1 regulating lipid metabolism were studied using two-dimensional electrophoresis,bioinformatics and plasma lipid biochemical detection technology. The result showed that 8 differential proteins（AHSG,KNG1,Serpin A3-1,ENSBTAG00000021729,Serpin A3-5,FAM208A,CTAGE5,and LOC511240）were identified. Bioinformatics predicted that there were 63 proteins interacting with these 8 differential proteins,and it was discovered that differential proteins and interacting proteins enriched in 11 biological pathways related to lipid metabolism,and the APOA1 of which enriched at the highest frequency in the 11 pathways. Accordingly,the significantly higher plasma level of APOA1 was detected in fat-1 transgenic cow than wild-type cow. Then,the plasma level of APOA1 was negatively related to the plasma level of low density lipoproten-cholesterol（LDL-C,r=-0.90）,whereas positively related to the ratio of high density lipoproten-cholesterol/total cholesterol（HDL-C/TC,r=0.69）. These results indicated the fat-1 gene regulated the lipid metabolism by mediating the expression of APOA1 in transgenic cow.

Key words: fat-1 transgenic cow, plasma protein, n-3 PUFAs, lipid metabolism

LIU Xin-feng^{1, 2}, DING Xiang-bin², WANG Yi-min², LI Xin², GUO Hong², LI Guang-peng¹. Study of Plasma Differential Proteins in fat-1 Transgenic Cow[J]. Biotechnology Bulletin, 2016, 32(9): 203-209.

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