Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (7): 107-113.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0067

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Research on Rhizosphere Soil Microbial Diversity of Two Typical Kinds of Disease in Yam

KANG Jie1 ,ZHANG Shu-yan1, HAN Tao1 ,SUN Zhi-mei2, LUO Tong-yang1   

  1. 1.Hebei Research Institute of Microbiology,Baoding 071051;
    2.Agricultural University of Hebei Province,Baoding 071000
  • Received:2017-02-08 Online:2017-07-11 Published:2017-07-11

Abstract:

For understanding the microbial community structure of soil rhizosphere with two typical diseases and clarifying the correlation between soil diseases and microbial diversity,high-throughput sequencing was applied to compare and analyze the microbial diversities of the rhizosphere with 2 typical diseases. As results,the population diversity and enrichment of bacteria in soil sample were the scab disease > CK > root-rot disease. While in fungi,the changes were more obvious,fungal diversity and enrichment in the soil with 2 diseases were higher than the control group. The species annotation showed that comparing with the control group,the bacterial species of different treatments were almost the same,but just the proportion of each were not identical. There were some specific fungal species which may be related to disease occurrence,and variation of proportion was significant,indicating that the changes of soil microbial structure affect the happening of the disease in a certain extent.

Key words: soil, microbial diversity, high-throughput sequencing, disease