Isolation and Identification of an Imazethapyr-degrading Bacterium，and Optimization of Biodegradation Conditions

doi:10.13560/j.cnki.biotech.bull.1985.2017-0076

Abstract

Abstract: This work aims to isolate and identify the strain IM9603 degrading herbicide imazethapyr，and optimize the degradation condition. IM9603 was identified as Brevibacterium sp. based on morphological，physio-biochemical characteristics，and analysis of 16S rDNA. Response surface methodology Box-Behnken was applied to optimize the degradation conditions. As results，strain IM9603 degraded 93.08% of the initial 50 mg/L supply of imazethapyr under the optimal condition（pH5 and 26.2℃）within 7 days，which was its best degradability. The isolated imazethapyr-degrading strain was Brevibacterium epidermidis，and presented high degradability to imazethapyr，suggesting that is owns a great potential in the environmental rehabilitation caused by imazethapyr.

Key words: imazethapyr, biodegradation, Brevibacterium sp., response surface methodology

Lü Fan-yang, XU Ze-hua, MAO Xiao-jie, SUN Jian-guang. Isolation and Identification of an Imazethapyr-degrading Bacterium，and Optimization of Biodegradation Conditions[J]. Biotechnology Bulletin, 2017, 33(10): 163-168.

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